MiR-381-3p Suppresses Cervical Cancer Proliferation And Metastasis By Down-regulating FGF7

Objective: This study directed at elucidating the molecular mechanism of miR-381-3p,which may improve the pathological study of cervical cancer and provide new theories and ideas for the treatment of cervical cancer.Methods: Microarray analysis screened out differentially expressed genes and q RT-PCR confirmed the low expression of miR-381-3p.Micro RNA base predicted the target gene and dual-luciferase reporter assay verified the direct target relationship.Then,western blot assay attested protein expression of target gene FGF7.Colony formation assay detected the cell proliferation and flow cytometry tested cell cycle and apoptosis.Transwell migration/invasion assay finally examine influences of miR-381-3p and FGF7 on cell migration and invasion.Results: MiR-381-3p was low expressed and FGF7 was high expressed in cervical cancer cells.MiR-381-3p and FGF7 had a direct target relationship and a negative correlation.MiR-381-3p could suppress FGF7 expression,resulting in proliferation,migration and invasion inhibition,cell cycle retardation and apoptosis acceleration.Conclusion: MiR-381-3p reversely regulated FGF7,resulting in tumor cells proliferation,migration and invasion inhibition,cell cycle retardation and apoptosis acceleration.