Effects Of Wutou Injection On NO,iNOS In Synovial Fluid And Col-2 In Articular Cartilage Of Rabbit OA Model

Objective: Through the use of Wutou injection to intervene in the experimental study of OA model rabbit arthritis,the changes of NO,iNOS content and the expression of Col-2 in articular cartilage were compared.The effects of NO,iNOS content and the production of Col-2 in articular cartilage were observed and the intervention of Wutou injection on OA was explored.The mechanism provides a theoretical basis for the clinical application of Wutou injection in the treatment of OA.Method:50 male New Zealand rabbits were randomly divided into blank group,model group,sodium hyaluronate group,Zhengqingfeng Tongning group and experimental group,with 10 rabbits in each group.In addition to the blank group,the mixed solution of 2%(w/v)papain and 0.03mol/L L-cysteine(2:1)was injected into the right posterior chamber of the right posterior chamber after 1st,3rd and 5th day anaesthesia,and the amount of injection was 0.1m L/kg.After the success of the model,each group was injected into the joint cavity for 4 times in 1st,4th,7th and 10 th day,the sodium hyaluronate group was given Sodium Hyaluronate Injection,the Zhengqingfeng Tongning group was given Zhengqingfeng Tongning Injection,the experimental group was given aconitas injection,and the model group was given saline.After 1 weeks of feeding,the 5 groups of rabbits were killed by air embolism,the articular fluid and the cartilage tissue of the tibial plateau were taken.The content of iNOS and NO in the articular fluid was detected by Elisa.The expression of Col-2 in cartilage tissue was detected by immunohistochemistry.Result: Compared with the blank group,the contents of NO and iNOS in the synovial fluid of the model group increased significantly(P<0.05),and the difference was statistically significant.Compared with the model group,the content of NO and iNOS in rabbit joint solution in sodium hyaluronate group was lower than that of model group(P<0.05),and the content of NO and iNOS in the joint fluid of the rabbits of Zhengqingfeng Tongning group decreased significantly(P<0.05).The content of NO and iNOS in the joint fluid of the hum injection group decreased significantly(P<0.05),and the differences were statistically significant between the groups.Compared with sodium hyaluronate group,the content of NO in the joint fluid of the Wutou injection group increased(P<0.05),and the iNOS content decreased(P<0.05),the difference was statistically significant.Compared with the Zhengqingfeng Tongning group,the content of NO in the joint fluid of the Wutou injection group decreased(P<0.05),the difference was statistically significant.Compared with the blank group,the expression of Col-2 in articular cartilage of model group was significantly decreased(P<0.05),the difference was statistically significant.Compared with the model group,the expression of Col-2 in articular cartilage of rabbits with sodium hyaluronate increased significantly(P<0.05),and the expression of Col-2 in articular cartilage of rabbit joint cartilage increased significantly(P<0.05),and the expression of Col-2 in articular cartilage of Wutou injection group increased significantly(P<0.05),and the differences were statistically significant in each group.Compared with the sodium hyaluronate group,the expression of Col-2 in the articular cartilage of the Wutou injection group decreased(P<0.05),and the difference was statistically significant.Compared with the normal Zhengqingfeng Tongning group,the expression of Col-2 in the articular cartilage of the Wutou injection group increased(P>0.05),the difference was not statistically significant.Conclusion: Wutou injection can reduce the expression of iNOS activity in synovial fluid of OA model rabbits,inhibit the production of NO in synovial fluid,and then inhibit the inflammation of joints and apoptosis of chondrocytes,promote cell proliferation and cartilage repair;Wutou injection can Increasing the expression of Col-2 in articular cartilage of rabbits with OA model may be related to the inhibition of degradation of cartilage matrix and play a protective role for articular cartilage.