Effect Of Helicobacter Pylori On LR11 And Arteriosclerosis Related Risk Factors In D-HAEC

Objective:To investigate the effect of Helicobacter pylori(HP)outer membrane vesicles(OMVs)on the diseased aortic endothelial cells in diabetic type II(D-HAEC),to further reveal the Helicobacter pylori infection of the occurrence and development mechanism of arteriosclerosis in(Type 2 diabetes mellitus,T2DM)to provide experimental data.Method:The HP strain(ATCC43504)was expanded in vitro.After the supernatant was collected the outer membrane vesicles were collected and examined by electron microscopy to verify that the outer membrane vesicles were successfully extracted.In vitro culture of D-HAEC,adding appropriate concentrations of outer membrane vesicles to cells after plating and co-cultivating for 24 hours.CCK-8 was used to perform cell counting assay,Finally,real-time relative quantitative PCR was used to detect the expression of human primers including IL-1?,IL-6,LR11,MCP-1,TNF-a and VCAM-1.Result:1.HP-OMVs stimulation increases expression of LR11 and arteriosclerosis-related risk factors in D-HAEC.2.Adenosine cyclase(AC)inhibitor SQ22536 inhibited the expression of LR11 and arteriosclerosis-related risk factors in the HP-OMVs positive group.3.HP-OMVs promote the growth of D-HAEC,and the number of cell growth increases with increasing concentrations.Conclusion:1.HP-OMVs carry its virulence factors acting on D-HAEC to promote the expression of LR11 and arteriosclerosis-related factors.2.SQ22536 inhibits the expression of LR11 and arteriosclerosis-related factors in the HP-OMVs positive group by altering the cAMP level.3.HP-OMVs promoted the proliferation of D-HAEC and showed a positive correlation.