The Effect Of LncRNA MEG3 On Apoptosis Of Pancreatic Acinar Cells In Acute Pancreatitis And Its Clinical Significance

Objectives:To explore the clinical significance of RNA-MEG3(lncRNA-MEG3,lncR-MEG3)in acute pancreatitis and the effect of its homologous analogue LncR-RGD1566401 on apoptosis of pancreatic acinar cells(AR42J)in rat acute pancreatitis model.Methods:LncR-RGD1566401 is a maternal blotting gene located in rat chromosome 6q32,with a length of 1,915 bp,and it is a homologous analogue of maternal expression gene 3(MEG3).The GFP LncR-RGD1566401 overexpressed Lentivirus and special LncRNA Smart Silencer were designed and transfected into AR42 J repectively for 24 h.The apoptosis of AR42 J in different groups was established by inducing cells with 100 nmol/L Caerulin for 24 h.The flow cytometry was used to detect the apoptosis of AR42 J in different groups.Real time RT-PCR was used to detect the expression level of lncR-RGD1566401 in AR42 J cells of different groups after transfection.The expression of Caspase3 and P53 was detected by western blot.In this retrospective study,63 patients of acute pancreatitis were consecutively included between Jun 2016 and Jun 2017,including 21 cases of mild acute pancreatitis,severe acute pancreatitis in 42 cases.All patients collected venous blood in 24 hours after admission.Real time RT-PCR was used to detect the expression level of lncR-MEG3 in acute pancreatitis.To analyze the variability among lncR-MEG3?C-reactionprotein(CRP)and D-Dimer in different groups.Using SPSS 22.0 software for statistical analysis,and draw the ROC curve.Results:The apoptosis rate [(19.34±0.54)%] of Caerulin-induced AR42 J elevated compared with the control group[(7.23±0.33)%].The expression of lncR-RGD1566401 in the AR42 J cells was increased by(5.43±0.31)times in the experimental group after dealing with Caerulin and the level of Caspase-3 and P53 were increased obviously.LncR-RGD1566401 level was elevated by(8.24±0.22)times after AR42 J cells were transfected with lenti-LncRNA as compared with the vehicle groups(p<0.05).The AR42 J cells showed more tendency to apoptosis [(38.20±0.37)%] than the vehicle groups[(22.60±0.96)%,p<0.05] and the level of Caspase-3 and P53 were increased obviously.The AR42 J cells that were transfected with Silencer-LncRNA expressed the lower level of lncR-RGD1566401 about [(0.54±0.14),p<0.05]times and less apoptosis [(13.60±0.33)%] than the Silencer-NC groups [(19.60±0.46)%,p<0.05].The level of lncR-MEG3 in MAP group is significantly increased compared with SAP group(p<0.05).The level of CRP and D-Dimer in SAP group is increased obviously compared with MAP group(p>0.05).Combined detection of lncR-MEG3,CRP and D-Dimer in prognosis of severe acute pancreatitis positive rate is higher than detection of CRP and D-Dimer or single positive rate.Conclusion:1.LncR-RGD1566401 is associated with the apoptosis of AR42J;2.Up-regulation of LncR-RGD1566401 can promote the apoptosis of AR42 J,while down-regulation of LncR-RGD1566401 can inhibit the apoptosis of AR42J;3.Patients with MAP have a higher expression level of circulating lncR-MEG3 than SAP.4.Combined detection of lncR-MEG3,CRP and D-Dimer in prognosis of severe acute pancreatitis positive rate is higher than detection of CRP and D-Dimer or single positive rate.5.The level of lncR-MEG3 can be a potential biomarker related to inflammation and used to diagnose SAP from AP.