A Preliminary Study Of Plasma Exosomal MiR-1273f As A Marker For Diagnosis Of Systemic Myasthenia Gravis

Objective: To detect plasma exosomal miRNA expression in patients with systemic myasthenia gravis and compare it with age-and sex-matched healthy controls to explore plasma exosome-derived miRNA as a marker for diagnosis of systemic myasthenia gravis possibility.Method: The patients with systemic MG diagnosed in the Department of Neurology clinics and wards of our hospital from December 2016 to October2017 were selected as the experimental group.The healthy persons matched with age and sex were selected from the control group.All the blood samples were collected and tested.Using ExoQuick Precipitation(separation method),transmission electron microscopy,plasma exosomes were isolated and identified,RNA was extracted,and sent to Guangzhou Ruibo Biotechnology Co.,Ltd.for deep sequencing.Based on deep sequencing results,the expression of plasma exosomal miRNA in systemic MG patients was analyzed and compared with the healthy control group.Bioinformatics analysis was used to screen for differences in plasma expression and associated with the pathophysiology of MG.Somatic miRNAs.RT-qPCR and other techniques were used to verify the expression of the key exosome-derived miRNAs screened.Results:The results of deep sequencing showed that exosome-derived miR-6843-3p,miR-23a-5p,miR-1273f,and miR-3182 were up-regulated in systemic MG compared with healthy controls.Plasma exosomes MiR-610,miR-3667-5p,miR-345-3p,miR-7641,miR-106A-5P,miR-138-5p,and miR-7-5p were all down-regulated.Bioinformatics analysis revealed that plasma outside the PCR validation found that miR-3182 and miR-6843 in the difference between the two groups was not statistically significant.miR-1273f miR-3173 f,miR-3143 and MG pathogenesis.The expression of systemic MG patients was higher than that of healthy controls(P < 0.05).Conclusion:Plasma exosome-derived miR-1273f may serve as a potential marker for the diagnosis of systemic MG.