| ObjectiveThis research aimed to study the demineralization and remineralization of dental hard tissue by the four components of gallic acid,methyl gallate,ellagic acid,and catechins in Geranium strictips.Compared with positive control group,to provide theoretical and experimental basis for clinical application of active compositions in Geranium strictips.Methods1 Using double dilution method to measure MIC of gallic acid,methyl gallate,ellagic acid,and catechins against Streptococcus mutans and Actinomyces viscous.2 Study four compositions of Geranium strictips on enamel in vitro2.1Using Micro indentation hardness to measure the microhardness of bovine incisors of four compositions and mixtures.2.2 Using Automatic potentiometric titratior to observe the ability of four compositions and mixtures to resist enamel demineralization and promote remineralization.2.3 Using atomic absorption spectrophotometer and ultraviolet spectrophotometer to measure the outflow of enamel calcium and phosphorous of four compositions and mixtures.2.4 Using scanning electron microscopy(SEM)to observe enamel surface morphology after remineralization of four compositions and mixtures.3 Study four compositions of Geranium strictips on dentin in vitro3.1 Using polarizing microscope and laser scanning confocal microscope to observe the the degree of caries and fluorescence of dentin remineralization of four compositions and mixtures.3.2 Using enzyme standard instrument to observe the effects of four compositions and mixtures on dentin collagen.Results1 Determination of MIC: the MIC of gallic acid and methyl gallate against Streptococcus mutans and Actinomyces viscous were 4.00mg/mL;The MIC of ellagic acid and catechins against Streptococcus mutans and Actinomyces viscous were1.00mg/mL.2 Study four compositions of Geranium strictips on enamel in vitro2.1 Micro hardness test: compared with the negative control group,the hardness before and after remineralization of experimental groups were different(P<0.05).Compared with the positive control,the hardness before and after remineralization of experimental groups had no difference(P>0.05).Two pairs of experimental groups were compared: mixtures,methyl gallate,ellagic acid and catechins were stronger than that of gallic acid(P<0.05).2.2Acid demineralization and remineralization experiments: compared with the negative control group,experimental groups had significant different ability in antiacid demineralization(P<0.01).Compared with positive control group,there was no difference in acid-demineralization ability of experimental groups(P>0.05),while the mixture group had stronger resistance to acid demineralization than that of positive control group(P<0.01);Two pairs of experimental groups were compared: mixtures,methyl gallate,ellagic acid and gallic acid were stronger than that of catechins(P<0.05).Compared with the negative control group,the ability of remineralization of experimental groups were different(P<0.05).Compared with positive control group,the remineralization ability of methyl gallate group and mixture group were stronger(P<0.05).Catechins group was weaker than positive control group(P<0.05);Gallic acid group and ellagic acidgroup had no difference with positive control group.(P>0.05);Two pairs of experimental groups were compared: mixtures and methyl gallate were stronger than that of gallic acid,ellagic acid and catechins(P<0.05).2.3The release value of calcium,phosphorus ions in demineralization solution: From the results of calcium ion efflux,experimental groups had statistically significance compared with the negative control group(P<0.01).Compared with the positive control group,the ability to inhibite calcium of ellagic acid group was small(P<0.05),and there was no statistical significance in other groups(P>0.05);.The outflow capacity of gallic acid,ellagic acid and gallic acid group inhibited calcium and phosphorus were less than the positive control group(P<0.01).Two pairs of experimental groups were compared: mixtures,methyl gallate,gallic acid and catechins were stronger than that of ellagic acid(P<0.05).2.4 Scanning electron microscope observation: there was a large amount of crevices on the surface of the enamel,and minerals decreased.In the negative control group,a small amount of sediments formed and the crevices decreased.The surface of enamel treated with gallic acid methyl ester was uneven,with some dense sediments.The surface of enamel treated with ellagic acid,catechins,and mixtures had a largenumber of crystal deposits with different microscopic morphology,but its structure was not dense enough.3 Study four compositions of Geranium strictips on dentin in vitro3.1Polarizedlight microscope observation: compared with the negative control group,the lesion depth of the experimental groups were significantly shallower.Among them,negative birefringence remineralization bands were observed in the lesion sites of the positive control group,the gallic acid methyl ester group,the ellagic acid group,and the mixture group.3.2 Laser scanning Confocal microscope observation: Compared with the negative control group,the total fluorescence and average fluorescence of experimental groups were different(P<0.05).Compared with the positive control group,the total fluorescence and average fluorescence of gallic acid was weaker(P<0.05).There was no difference between the other groups and the positive control(P>0.05).Two pairs of experimental groups were compared: mixtures,methyl gallate,ellagic acid and catechins were stronger than that of gallic acid(P<0.05).3.3Hydroxyproline concentration in remineralization solution: compared with the negative control group,there was difference in inhibiting degradation collagen between experimental groups(P<0.05).Compared with the positive control group,the mixture group inhibited collagen degradation stronger(P<0.05).There was no difference between other groups and positive control group(P>0.05).Two pairs of experimental groups were compared: mixtures,methyl gallate and ellagic acid were stronger than that of gallic acid and catechins(P <0.05).Conclusions1 The ability to inhibit the growth of Streptococcus mutans and Actinomyces virens,Ellagic acid and catechins were stronger than that of gallic acid and methyl gallate.2 Methyl gallate,catechins and mixtures had the effect of improving the surface hardness of tooth enamel.3 Gallic acid,methyl gallate,ellagic acid,catechins,and mixtures had the ability to resist acid demineralization.The mixtures,methyl gallate,gallic acid and ellagic acid are more effective than catechins.4 Gallic acid,methyl gallate,ellagic acid,catechins and mixtures had the ability to promote enamel remineralization.The methyl gallate,ellagic acid and mixtures had a stronger promoting effect.5 Gallic acid,methyl gallate,ellagic acid,catechins and mixtures had the effect ofinhibiting the calcium and phosphorus release of enamel.The catechins and mixtures had a stronger promoting effect.6 Methyl gallate,ellagic acid,catechins and mixtures had the effect of promoting the remineralization of dentin.The methyl gallate,ellagic acid and mixtures had a stronger promoting effect.7 Methyl gallate,ellagic acid and mixtures had the effect of inhibiting the degradation of dentin collagen. |
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