| Objective: To explore the influence of gastrodin on IL-10,JAK2 and STAT3 in hippocampus of epileptic rats induced by pentylenetetrazol and the role of the IL-10 pathway in epilepsy.Methods :50 adult male Wister rats were randomly divided into 5 groups: normal control group(NC group),epilepsy model group(EP group),low doses of gastrodin +EP Group(GE1 group),medium doses of gastrodin +EP group(GE2 group),high doses of gastrodin +EP group(GE3 group).EP group and GE groups injected sub-eclampsia PTZ doses(35mg/kg)by intraperitoneal to preparate chronic Epilepsy model;GE groups respectively injected 4.0,6.0,8.0mg/kg gastrodin by intraperitoneal after injection of PTZ 60min;the control group intraperitoneal inject equivalent saline to replace PTZ and the middle dosage of gastrodin.The time of injection was constant everyday.Observed rats one hour after each injection.At the same time,the epileptic seizures in each group were recorded,including the attack grade and the incubation period(from the intraperitoneal injection of PTZ to the onset of seizures,in seconds);duration(from onset of seizures to termination,in seconds),and death.In the 28 d,the rats in each group were killed with Urapidil,and the bilateral hippocampus were quickly preserved in liquid nitrogen.Detect the expression of hippocampal IL-10,STAT3,JAK2 mRNAs by RT-q PCR,and detect the proteins by Western blot.Results:(1)Behavioral observation in rats: The control group did not observe epileptic seizures;in 3d,30.0% rats in the model group began to appear I-?level epileptic seizures,the serious period appeared in 21d~28d,the success rate of model making is 80.0%;In 3d,13.3% rats in the intervention groups began to appear I-level epileptic ?seizures,the severe period appeared in 25d~28d.In the NC group,a rat died in the first day,and in the EP group a rat died in the 28 th days,no rats died in the other groups.All the rats in the PTZ,GE1,GE2 and GE3 group reached the ignition standard at the 18 d,showing a pronounced nod,facial and limb convulsions,erect vertical tail or fall or even systemic ankylosis.Compared GE2 group with EP group,the incubation period of onset was significantly prolonged,the duration is shorter and attack strength is lower.Compared with EP group,in the GE2 group,the duration of attack was significantly decreased(p<0.01),latent time was significantly prolonged(p<0.01),the attack grade was significantly decreased(p<0.05),and the inhibition was most obvious.There was no significant difference in the intensity,duration and latency of seizures between GE1 group and EP group.And no significant difference of convulsion intensity between GE3 group and EP group,but the duration of attack and latency time were significantly decreased(p<0.05).Between GE1 group,GE2 group and GE3 group,the attack intensity,duration and latent time had no significant difference.(2)Relative expression of IL-10,STAT3 and JAK2 mRNAs in hippocampus of each group: Compared with NC group,the expression of STAT3 and Jak2 mRNAs in EP group was significantly higher(p<0.05),and the IL-10 mRNA significantly decreased(p<0.05);The IL-10 mRNA expression level in GE2 group was significantly higher than that in EP group(p<0.01);Compared with EP group,the Stat3 mRNA expression level in GE2 group and GE3 group were significantly decreased(P<0.01).Compared with EP group,the Jak2 mRNA expression level of GE2 group significantly decreased(p<0.05).(3)Relative expression of IL-10,STAT3 and JAK2 proteins in hippocampus of each group: Compared with NC group,in the EP group IL-10 protein expression significantly decreased(p<0.05),STAT3 protein expression was significantly higher(p<0.01),and Jak2 protein was significantly higher(p<0.05);Compared with EP group,in the GE2 group IL-10 protein expression significantly increased(p<0.05),Jak2 protein was significantly lower(p<0.01).and the STAT3 and Jak2 protein expression significantly decreased in GE3 group(p<0.05).Conclusions: Intraperitoneal injection of the subconvulsions dose of PTZ(35mg/kg)can induce chronic epileptic seizures in rats,and gastrodin can control the seizure of epileptic rats induced by PTZ,and medium doses of gastrodin(6.0mg/kg)inhibitory effect is most significant.The expression level of IL-10 in hippocampus of epileptic rats was decreased,and the expression level of IL-10 was increased after gastrodin intervention,while the expression level of STAT3 and JAK2 in hippocampus of epileptic rats increased,and the expression level of STAT3 and JAK2 was decreased after gastrodin intervention.It is suggested that gastrodin can increase the expression of IL-10 and reduce the expression of STAT3 and JAK2,which may play an antiepileptic function through regulating JAK2/STAT3 signaling pathways by IL-10. |
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