Variation In Expression Of α-SMA After Cerebral Ischemia Reperfusion In Diabetic Mice

Objective To discuss the alteration of pericyte in hyperglycosemia cerebral ischemia reperfusion damage by performing the expression changes of α-smooth muscle actin(α-SMA)in hyperglycosemia cerebral isehemia reperfusionin damage of mice.Methods The model of diabetes mice was set up using Alloxan.Silk thread was applied to barraging the middle cerebral artery(MCA)occlusion,then the experimental models were established.Using ethology,immunohistochemistry,Western blot and qRT-PCR,The otherness of the pericyte’s number of cerebral cortex of frontal lobe and the expression of α-SMA of diabetes hyperglycosemia cerebral ischemia reperfusion group(hyperglycosemia & ischemia group)and normoglycemia cerebral ischemia reperfusion group(ischemia group)at 1 hour after cerebral ischemia,24 hours after reperfusion were observed and compared.Results Relative to the blank control group,the immunohistochemistry as well TTC staining showed that gently edema occurred after reperfusion for 24 hours,the number of pericyte were increased obviously in ischemia group(P<0.05);The pathology change was more significantly in hyperglycosemia & ischemia group than that in the ischemia group after reperfusion for 24 hours.Cerebral edema was aggravated remarkably,at the same time,the number of pericyte were increased massively(P<0.05).qRT-PCR and Western blot showed that the expression of α-SMA was higher in hyperglycosemia & ischemia group than that in ischemia group(P<0.05).Conclusion Diabetes hyperglycosemia cerebral ischemia reperfusion can aggravate cerebral damage,reinforce the expression of a-SMA and increase the number of pericyte.