Magnesium Sulfate Through NF-?B/ICAM-1 Pathway To Protect Radiation-induced Brain Injury

Objective:Study magnesium sulfate effects on nuclear factor-kappa B and intercellular adhesion molecule-1 expression in C57BL/6J mice radiation-induced brain injury,and to explore the protective molecular mechanism.Methods:1?C57BL/6J mice were subjected to whole brain irradiation with 5 MeV electron beam 10 Gy,15 Gy and 20 Gy respectively,and the optimal dose of radiation-induced brain injury was established by HE staining.2?HE staining method and immunohistochemical staining method to establish the optimal concentration of magnesium sulfate on the protective effect of radiation brain injury.3?NF-?B and ICAM-1 mRNA expression in brain tissue of wild-type mice and P50-/-mice was detected by real-time quantitative PCR(QRT-PCR)to detect magnesium sulfate on that influence.4?NF-?B and ICAM-1 protein expression in brain tissue of wild-type mice and P50-/-mice was detected by immunohistochemical staining and enzyme-linked immunosorbent assay.5?Western blot assay were used to detect the effect of magnesium sulfate on COX-2 protein expression.Results:1?With the single fractionated dose of 20 Gy by electron beam(5 MeV)can successful establish the mice model of RBI in wild and P50-/-mice.2?HE staining and immunohistochemical staining results showed 10%300 mg/kg magnesium sulfate is the most suitable concentration.3?Magnesium sulfate can inhibit NF-Kappa B and ICAM-1mRNA and protein expression after irradiation,so as to alleviate the symptoms of radiation-induced brain injury.4?Magnesium sulfate can inhibit ICAM-1mRNA and protein expression in P50-/-mice after irradiation.5?Magnesium sulfate can reduce COX-2 protein expression after irradiation in wild-type mice.Conclusion:This paper from wild-type and P50-/-mice both confirms that magnesium sulfate can reduce the NF-Kappa B and ICAM-1mRNA and protein expression,as well as improve the pathology of radiation brain injury,the experiment results showed that the protective effect of magnesium sulfate on radiation-induced brain injury can through NF-?B/ICAM-1 molecular mechanism of this signaling pathway.