Effects Of Fructo-oligosaccharide And Fructose On Intestinal Microflora In IBS-D Rats

Objective:To establish a more effective and easier model of irritable bowel syndrome with diarrhea(IBS-D)in rats.Using oligosaccharides and the fructose to interfere with IBS-D rats to research the effect on the observation data and intestinal flora of IBS-D rats.Methods:The rats were randomly divided into four groups:the normal without any intervention(N),The Folium Cassiae only(F),Limbs of the rats were tied up with cotton rope,binding on the cages(S),The rats were fixed in 50ml centrifuge tubes(L).The normal group was given the same volume of normal saline.We observed the status,the body weight,food-intake and defecation of each groups.The model of 35 ICR female rats were made using the best modeling method.Then they were randomly divided into control,low,middle,high dose fructo-oligosacc-haride and fructose groups.The gavage dosage of fructo-oligosaccharide were(1.5g/Kg·BW),(0.5g/Kg·BW),(0.25g/Kg·BW),and the gavage dosage of fructose were(8g/Kg·BW),(5g/Kg·BW),(2g/Kg·BW).Both gavage were given for consecutive 21days,while the IBS control group rats were given same volume of saline.The fresh deces of rats collected on the last day,which were used for the high-throughput sequencing of 16SrDNA to analyze the variation of the relative abundance of bacteria.Results:1.Screening of modeling methods of IBS-D rats.Group S and group L can reproduce IBS-D in rats successfully.Group L was the best among these four groups for its simple and stable.2.Comparison of intestinal flora between IBS-D rats and control rats.The content of lactobacillus in the IBS group was lower than that in the control group,and the content of bifidobacterium in the IBS group was significantly lower in the control group(P<0.05).3.Effects of oligosaccharide on IBS-D rats.Compared with the IBS control group,there was a smaller amount of Actinobacteria in medium oligosaccharide group(P<0.05).The differences in the contents of Lactobacillus were not statistically significant while the content of Bifidobacterium in high oligosaccharide group was significantly higher than that in the IBS control group(P<0.05).4.The effect of fructose on IBS-D rats.The ratio of firmicutes and bacteroides(F/B)was larger in low fructose group(P<0.05).Compared with the IBS control group(P<0.05).The concentration of Lactobacillus in the high fructose group was the lowest,and the abundance content of the control group was the largest in the IBS control group(P>0.05).5.The self-healing effect of IBS-D ratsCompared with IBS group,firmicutes had lower adundance in IBS control group while the abundance of Bacteroidetes increase.The abundance content of lactobacillus in the IBS group was higher than that in the IBS control group(P<0.05).Conclusion:1.The restriction of centrifuge tubes +intragastric administration with 0.5g/ml folium cassiae was the safest and easiest way to establish IBS-D mouse model in three methods.2.There was a difference of microorganism intestinal flora between IBS group rats and the control group rats.3.Oligosaccharide could increase the fecal stool rate of IBS-D rats.4.The intestinal flora of IBS-D rats was disorganized with the increase of fructose concentration,and fructose could aggravate the progress of IBS disease.5.With the passage of time,the content of probiotics in the intestinal flora of IBS-D mice decreased,and the content of harmful pathogenic bacteria increased.The intestinal flora of IBS-D mice did not heal over time.