Establishment Of Immunochromatographic Strip Test For Diagnosis Of Trichinellosis

Trichinellosis is one of most important zoonosis that poses a threat to public health and also causes losses to the aquaculture industry.Therefore,it is necessary to develop detection rapid and sensitive method for the detection of trichinellosis which provides strong support for the prevention of Trichinella infection.The ES antigen was obtained from muscle larvae RPMI1640 medium,and the antibodies in the positive serum from pigs with various worm burdens were detected.The obtained result can be used to test the accuracy of the test strips for the detection of Trichinella spiralis.At the same time,the ES antigen from muscle larvae was used as diagnostic antigen.Through the selection of various solid-phase materials and optimization of reaction system and reaction conditions,a immunochromatographic strip based on fluorescent microspheres labeled goat anti-porcine IgG for the detection of Trichinella spiralis was established.ES antigen was obtained from muscle larvae at the concentration of 2 mg/ml.The NC membrane Millipore 135 with 1 mg/ml ES antigen and 0.625 mg/ml rabbit anti-goat IgG,conjugate pad Ahistrom8964 with time-resolved fluorescent microsph-ereabeled goat anti-pig IgG,XQ-Y8 sample pad,fluorescent black plate and absorbe-nt paper H5072 comprised Trichinella Fluorescence Immunochromatographic strip.The strip can detect the anti-Trichnella spiralis serum at least 1:100 dilution degree,and not react with the anti-Taenia solium,anti-clonorchis sinensis serum,etc.The results showed that the sensitivity of the strip was lower than an order of that of OIE standard enzyme linked immune method,but the trend of the levels of anti-Trichinella spiralis antibodies were consistent.In conclusion,this study established the immunochromatographic strip with goodspecificity,sensitivity and repeatability,which was able to detect the anti-Trichinella spiralis antibody in the pig serum rapidly and,could be applied in the field test.