| Objective: To establish a model of animal intoxication and secondary poisoning and determine whether the second generation of anticoagulant rodenticide can produce secondary poisoning,such as secondary poisoning,and the relationship between toxicity and dosage and heating.To provide a theoretical basis for the diagnosis and timely treatment of the clinical second-generation anticoagulant rodenticide poisoning.Methods: First,Rat model of bromadiolone poisoning was established.Selected 5 doses of poison: Group A: 2 mg/kg,Group B: 1 mg/kg Group,Group C: 0.75 mg/kg virus Group,Group D: 0.5 mg/kg Group,Group E: 0.1mg/kg Group,8 rats per group,feeding 21 days,observe the hemorrhagic symptoms and the time of death in rats.Calculate the mortality of the rats according to the death of the rats.Select the mortality rate as 50% of the dose to ensure that it can not only cause hemorrhage in the rat's bromadiolone poisoning but also survive in rats.Secondly,the experimental sampling time is selected.Six sampling time points were selected,and 66 rats were 0.5mg/kg doses,feeding 14 days,respectively,in the No.0,1,3,7,10,14 days after the infection of six rats abdominal aorta blood,check the poison detection and blood coagulation function,according to poison detection and the result of coagulation blood selected sampling time.Furthermore,the rat model of bromine enemy lung toxicity was established.Selected 5 doses: Group A for 0.5mg/kg,Group B for 2.5mg/kg,Group C for 10mg/kg,Group D for 50mg/kg,Group E for Blank Control,in each group of 20 rats,group A and B were low dose group,group C and D were large dosage group,Group E was blank control group,feeding for 5 days,observed the symptoms of rats,hemorrhage,The time of death,respectively,on the first day,the third day after the infection,on the fifth day,3 rats were randomly executed,respectively,the abdominal muscle division heating and the unheated group and thigh muscle group were tested,and the concentration of poison in the muscles of different parts,different doses and the heated rats were compared.According to the results of the TOX sample,the time and dose standard of the muscle required for two times of intoxication were selected.Finally,on the basis of establishing the model successfully,secondary poisoning experiments were carried out.To grind the muscle of the rat in the selected drug dosage and sampling time,after homogenate,divided into five groups: Group A: 0.5mg/kg third day heating abdominal muscles,Group B: 0.5mg/kg The third day did not heat abdominal muscle,Group C: 50mg/kg third day heating abdominal muscles,Group D: 50mg/kg The third day did not heat the abdominal muscles,Group E: Blank control,each group of 12,the above muscle irrigation stomach to the rats,feeding 3 days,on the first day and the third day,respectively,5 rats abdominal aorta blood check poison detection and blood coagulation function.Results: 1.The mortality rate of rats with different doses was calculated as 0.5mg/kg.2.The time range of abnormal coagulation function in rats induced by poisoning was 1-7 days after infection,after the infection,the abnormal degree of coagulation in the third-day rats reached the peak,and the coagulation function of the seventh rats was normal after the infection,and the concentration of the enemy lung in the blood was decreased after the poisoning,and the concentration of poison in the blood was up to a peak and the third day followed.3.Compare the results of the detection of abdominal muscle toxins in rats with different doses and at the same time,in rats with the same infection time,the higher the concentration of the virus detected in the muscle,the greater the concentrations of the bromine enemy in the muscles.Comparing the results of abdominal muscle toxicology in rats with the same drug dosage and different sampling time,we can see that the same drug-exposed rats,the concentration of the enemy lung in the muscle was detected first and then decreased.In the third day after the poisoning,the concentration of bromine enemy lung reached a peak in the muscle.Comparing the results of toxicological tests of the same drug dosage and the same time sampling in rats ' abdominal muscles and leg muscles,the same drug dose was found in the same exposure time.The concentration of toxicants in the heated abdominal muscles was higher than that in the unheated abdominal muscles.Compared with the results of toxicity test before and after abdominal muscle heating in rats with the same dose and at the same time,Abdominal muscles have higher concentrations of toxicants than leg muscles.4.Compared with the toxic concentrations in rats with low dose and high dose of secondary poisoning,it was found that the possibility of secondary poisoning in rats was small.Conclusion:1.The dose of 50% of rat bromodiolone mortality was 0.5 mg/kg;2.The higher the concentration of bromodiammine in rats,the greater the degree of poisoning in rats,and the higher the concentration of toxicants detected in rats' blood and meat.3.After bromindrosol poisoning in rats,the presence of bromindrosol in the body increased first and then decreased.4.Rats with bromidonol poisoning cause abnormal coagulation in rats;5.After the rats were bromatol poisoning,there was no significant correlation between the site and extent of hemorrhage and the concentration of bromodiamazole in rats.6.After the rats were exposed to bromadiolil,the distribution of poisons in different parts of the body of rats was different.7.Second-generation anticoagulant rodenticides are less likely to cause secondary poisoning;8.8.The second generation of anticoagulant killing rats was less likely to be poisoned by two times.9.Heating can significantly change the concentration of the bromine enemy lung in the muscle,reduce the total content of the bromine enemy lung. |
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