The Protective Effect Of Notoginsenoside For Bone Marrow Mesenchymal Stem Cells From Ischemic Injury

Objective To study the injury effect of thrombin to bone marrow mesenchymal stem cells(BMSCs),the changes of their cell activities and the protein expressions of ERK1/2 and p-ERK1/2 in vitro.And then intervention with notoginsenoside(PNS),to see whether the notoginsenside can protect bone marrow mesenchymal stem cells from the damage of thrombin.Methods BMSCs from SD rats were cultured in the whole bone marrow culture manner.Then the cells were passaged,purified and identified.Evaluated the concentration and time for cells by MTT method,then choose the BMSCs from the third to the eighth generations and devided in 6 groups randomly:PNS200 group,PNS100 group,PNS50 group,PNS20 group,injury control group and the normal control group.Detecting the activity of ALP,and the expression rates of ERK1/2?p-ERK1/2 in western blotting.Results 1.Compared with the normal control group,the injured group has a significant decreasing tendency of the ALP activity.The PNS intervention groups'ALP activity increasing compared with the injured group.there were no difference between the PNS intervention groups.2.The expressions of ERK1/2 has no significantly different in the six groups.The expression of p-ERK 1/2 in injured group has an obvious decreasing tendency compared with the normal control group,with statistically significant(P<0.01).The PNS intervention groups all have increasing tendencies of p-ERK1/2 expression compared to injured group,especially in 25 and 50 PNS groups(P<0.05).Conclution Certain concentration of thrombin can injure BMSCs,leading to a reduction of the ALP activity and the expressions of p-ERK1/2 in BMSCs.PNS has a protective effect for BMSCs of ischemic damage by acting ERK signaling system probably.