Effects Of Notoginsenoside Rg1 On Idiopathic Pulmonary Fibrosis

Background The causes and mechanisms of Idiopathic Pulmonary Fibrosis(IPF)are still unknown.Transforming growth factor-?1(Transforming Growth Factor-?1 TGF-(?1)is a key factor to fibrosis,because it can induce fibroblasts to myofibroblasts cells.Caveolin-1(Caveolin-1)is the main structural component of caveolae and iconic proteins,modifying signal transduction molecules and negative regulation of many signal transduction pathways and biological processes.Study confirmed that downregulation of caveolin-1 significantly reduces TGF-?1,cell signal transduction molecules(Mothers Against Decapentaplegic Homolog,Smad)and other negative regulatory effect on multiple signaling pathways such as pulmonary fibrosis and thus accelerate process.Objecive The purpose of the normal construction with bleomycin-induced IPF model SD rats,Panax Rgl on IPF explore the role and mechanism of action,providing experimental and theoretical basis for the IPF drug development and clinical prevention.Methods 70 clean healthy male SD rats weighing 220±10g,were randomly divided into sham group(Sham),model group(Model),model + positive control group(M + P),model ?notoginsenoside Rgl 18mg/kg group(M + NL),model + notoginsenoside Rgl 36mg/kg group(M + NM)and the model + notoginsenoside Rgl 72mg/kg group(M + NH),a total of six groups,normal control group of 10 animals other groups each of 12 animals each,the normal control group 2 animals died(n = 8),model group had four animals died(n = 8),M +NL and M + NM group have three animals died(n = 9),M + NH group 2 animals died(n ?10).Animal two days after modeling started medication,administered once daily,were administered orally.Sham group and model group were given an equal volume of saline(5ml/kg),M + P group were given prednisone(5 mg/kg),the model Rg1 notoginsenoside each dose group to give the corresponding dose notoginsenoside Rgl.When to continuous medication 7d,killed four animals in each group,and the remaining animals in each group were sacrificed after the next batch of medication to 28d animals,all animals placed in lung tissue after-40 ? preservation.Using Masson staining morphology and Elisa method for detecting ?-actin to evaluate the pharmacological effects of Panax Rg1 on IPF;using real-time quantitative PCR and Western blot technique to detect Panax Rgl on Caveolin-1 and transforming growth factor-?1(TGF-?1)affect mRNA and protein in order to clarify the mechanism of action.Result 1.Notoginsenoside Rg1 can reduce the increased fibrosis grade of IPF rat and the dose dependent was demonstrated.2.Notoginsenoside Rg1can reduce the increased expression of ?-SMA,the dose dependent growth inhibition was not demonstrated.3.Notoginsenoside Rg1 can down regulation TGF-?1 mRNA and protein of the IPF rat.the dose dependent growth inhibition was demonstrated.4.Notoginsenoside Rg1 can up regulation the expression of Cav-1 mRNA and protein of IPF rat.There was no demonstrated dose dependent.Conclusion Notoginsenoside Rg1 has a good anti-IPF effect,its mechanism of action related to up regulation the expression of Caveolin-1 mRNA and protein,down regulation the expression of TGF-?1 mRNA and protein.