| Objective Mycobacterium tuberculosis in the early fifties of the 20th century,along with some four anti-TB drugs,emerging,has been phased control.Since the 1940s,streptomycin used in TB treatment,and the subsequent first-line anti-TB drugs rifampicin,isoniazid,ethambutol applications,human control of TB have a breakthrough.However,due to a variety of factors were partially widespread abuse of antibiotics concurrent infection with HIV,the resurgence of tuberculosis,especially in developing countries such as India,China,is to become the hardest hit by MDR-TB.So explore new anti-TB imminent,Baicalin is a medicinal plant Scutellaria flavonoids,has studied a variety of effects,including anti-TB treatment,based on the current situation and the anti-TB drug resistance tuberculosis drug side effects,With this topic in vitro antibacterial test,study resistance gene KatG,InhA,ropB,embB fragment changed after baicalin role for combination therapy of tuberculosis explore new ideas,provide the basis of experimental data,and to lay the foundation for further clinical.Methods Choose single-resistant isoniazid,rifampicin,ethambutol strains each 15,first recovery using checkerboard method,different concentrations of baicalin 45 TB respectively with different concentrations of isoniazid,Lee rifampicin,ethambutol joint cross antibacterial test,TB save before taking action,and to save the bacteria do after use;around the same strain of the bacteria are lysed were performed,by PCR amplification of the lead iso KatG isoniazid-resistant,InhA gene,resulting in ropB rifampin resistance gene,resulting in embB ethambutol resistance gene,the PCR products were sequenced fragments observed resistant TB is easy to produce a joint baicalin changes in anti-TB drug resistance gene therapy fragments.Results After isoniazid,rifampicin,ethambutol cross joint antibacterial,results showed that baicalin with INH,RFP combined effect mainly as synergistic or additive,and the combined effect of EMB mainly as antagonistic;lead to isoniazid-resistant KatG,InhA gene,resulting in ropB rifampin resistance gene,resulting in resistance to ethambutol embB gene fragments,changes in the role after baicalin genes.Conclusions Joint susceptibility test results baicalin and INH,RFP,EMB showed that baicalin with INH,RFP combined effect mainly as synergistic or additive,and the combined effect of EMB mainly as antagonistic,indicating baicalin can partially reduce the INH,RFP resistance,reduce the required dose of the drug,reduced toxicity,avoid and delay the occurrence of resistance;mechanism portion of target genes associated with drug resistance,on the other hand may be associated with efflux pumps;baicalin EMB can not reduce the extent of drug resistance,but further increase its resistance to increase their dose,more likely to induce side effects,through this experiment did not find the target gene variations,should be further repeated studies,and further research of other target genes. |
