| Di-N-butylphthalate(DBP)is an important raw material of industrial manufacture,including the production of plasticizer,adhesives,spices and pesticide carrier etc.It is closely related with human life because it can not tightly combined with organic carriers and become ubiquitous environmental pollutant.It causes toxic effects on body health especially the male reproductive system through polluting air,water,soil and organism.Sulforaphane(SFN)is a kind of isothiocyanates,it is used in food industry,health care and medical fields because of its antioxidant and antitumor characteristics.The researchers in our lab had confirmed that SFN can reduce the toxic effect of Leydig cells induced by DBP in vitro.Here,we investigated the effects of DBP on the male reproductive system of pubertal mouse and explored the protective role of SFN.30 male C57BL/6J mice were randomly divided into 6 groups and treated with DBP and/or SFN for 2 weeks.Results showed that DBP significantly reduced anogenital distance(AGD)and testicular weight,sperm count and motility,plasma and testicular testosterone,and significantly increased the oxidative stress,sperm abnormalities,testicular cell apoptosis,while SFN supplementation ameliorated the same.After DBP stimulation,the level of transcriptional factor nuclear factor erythroid related factor 2(Nrf2)was adaptively increased together with its target genes,like heme oxygenase-1(HO-1)and NAD(P)H-quinone oxidoreductase(NQO1).Upregulation of Nrf2 by SFN not only restored the intracellular oxidative toxicity but also testosterone secretion and spermatogenesis in response to DBP.These findings indicate that SFN can attenuate DBP-induced reproductive damage in pubertal mouse through Nrf2 associated pathways. |
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