Investigation Of The Roles Of Long Noncoding RNA BX357664 In Progression Of Renal Cell Carcinoma

Background:Long non-coding RNAs(IncRNAs)are reported to serve as regulating role in carcinogenesis of various human malignancies.However,the function of IncRNAs and their underlying mechanism in renal cell carcinoma(RCC)is still unraveled.The aims of this study were to investigate the expression of lncRNA BX357664 in RCC and explore its function in RCC cell lines.Methods:Previous microarray analysis screened potential deregulated lncRNA.Real-time qualitative PCR(qRT-PCR)was used to confirm the deregulation of BX357664 in RCC.Ability of migration,invasion and proliferation in RCC cells were detected by cell migration and invasion assay,cell proliferation assay and cell cycle assay.Western blot identified the influence of BX357664 on epithelial-mesenchymal transition(EMT),MMP9 and TGF-beta 1/p38/HSP27 signaling pathway in RCC.Results:BX357664 was downregulated in RCC.After upregulation of BX357664 in RCC cells,malignant behaviors of cells were significantly inhibited.In addition,BX357664 could block EMT,MMP9 through inhibiting TGF-beta 1/p38/HSP27 signaling pathway.Subsequently,upregulating protein level of TGF-beta 1 with the present of BX357664 could rescue the malignant behaviors inhibited by BX357664 which indicated that BX357664 was attributed its inhibitory role to suppression of TGF-beta 1.Conclusion:We have revealed a novel IncRNA BX357664,which might-exhibit its inhibitory role in RCC metastasis and progression via block TGF-beta 1/p38/HSP27 pathway.