Study On Genetic Diversity Of Bacteria In Millet Sclerospora Graminicola And Analysis Of Endophytic Bacteria In Resistant Cultivars

Millet is the staple food crop in our country,we pay more attention to the quality and the yield in today's agricultural production.There are some reasons of affecting the production and the quality.One of the most important reasons is millet's diseases.So it is important to solve the problems.Sclerospora graminicola(Sacc).Schroeter is one of the main diseases that hinder the development of the millet industry.The Pathogenic bacteria of Sclerospora graminicola(Sacc).Schroeter is a special parasitic fungus,which is Sclerospora graminicola.In this study,it collects 128 samples of Sclerospora graminicola(Sacc).Schroeter from different millet-producing areas as the materials to study the genetic variability of Sclerospora graminicola(Sacc).Schroeter using PCR molecular detection technology.140 millet varieties from different areas is chosed as the test material.To appraise the antibody of Sclerospora graminicola(Sacc).Schroete,artificial inoculation techniques were used.And 4 highly resistant varieties and 5 susceptible varieties were selected from 140 test materials with resistant varieties.Using metagenomics technology,the endophytic bacteria of the resistant and susceptible millet varieties were analyzed.Get the following results:(1)The 28 Sr RNA sequence of white pathogenic bacteria will change with the region.According to the 28 Sr RNA sequence of the white bacterium in the Gen Bank database,7 pairs of special primers of Sclerospora graminicola(Sacc).Schroeter were designed,and the specific primer 28S-170 F2/28S-1530 R2 was sifted out,and 128 genomes DNA of samples was amplified by PCR.It can amplificate the target fragment of 1360 bp,then compare the results with the 28 S r RNA sequence.There are172 intraspecific polymorphic loci of the Sclerospora graminicola(Sacc).Schroete,including 1 Many variant sites,polymorphic sites are mostly the conversion between AG.92 haplotypes were detected in 128 samples.The SG6 haplotype is the best because it appeared the most frequently.Sclerospora graminicola(Sacc).in Shanxi,Guizhou,and Hebei contained the SG6 haplotype.(2)Investigation on the resistance grade of Sclerospora graminicola(Sacc).Schroeter.There are 140 millet varieties with Sclerospora graminicola(Sacc).Schroeter were investigated.It was found that there were 8 varieties with level one(high resistance)and 37 with leve lthere(disease resistance).There are 77 resistant varieties with level five(lightly susceptible)and 18 resistant varieties with level seven(susceptible).There are no resistant varieties with level nine.(3)Adopting metagenomics technology to analyze the community and function of the endophytic bacteria of the disease-resistant and susceptible varieties of Sclerospora graminicola(Sacc).Schroeter.The communities of endophytic bacteria in different parts and in different varieties is different.The gene sequence obtained by metagenomic is compared with the KEGG database.It shows that the functional genes are mainly distributed in the Membrane Transport of Metabolism and Amino Acid Metabolism.Comparing them and it is found that the microbial community functionality of the two groups G3(stem)and G5(heading ear)is higher than other groups.The functionality of G1(leaf),G4(leaf sheath),G6(mature ear)is smaller than other groups.This study and analyzed the genetic diversity of Sclerospora graminicola(Sacc).Schroeter from different sources of geographic,and identified the resistance of Sclerospora graminicola(Sacc).Schroeter with different varieties of millet,selected resistant varieties and analyzed by metagenomics technology their endophytic bacteria in different parts and different periods.It provides some basic information for breeding of millet.