Preparation Of Artemisinin Nano-copper And Its Antibacterial Activity Research

In the early 1920 s,antibiotics were used as a special medicine for the treatment of pathogenic microorganisms.However,irrational use of antibiotics cause pathogenic microorganisms resistant to antibiotics.This research was mainly focused on synthesis of artemisinin-nano-copper,which improved the shortcomings of easy aggregation,low bioavailability,and poor solubility when the artemisinin and nano-copper were used separately.Moreover,artemisinin-nano-copper also shows better antibacterial properties.The methods and results used to test the characterization and antibacterial properties of artemisinin-nano-copper as shown below:1.Synthesis of artemisinin-nano-copper.Chitosan and L-ascorbic acid were used as modified protective agent and reducing agent,respectively.Then add artemisinin for reduction reaction.The temperature was controlled between 60-100 ℃,reaction time was set(30 min-4 h),chitosan dissolution conditions(deionized water or 1% acetic acid solution).After testing the order of addition of each substance(copper source and artemisinin are added The reaction sequence),the ratio of each substance(the mass ratio of L-ascorbic acid,copper sulfate,and artemisinin)and the speed pf precipitation,drug sensitivity and transmission electron microscopy results.The results shown artemisinin-nano-copper can be successfully synthesized at 80 ℃ with 30 min reaction.Chitosan was directly dissolved in deionized water.The copper sulfate solution need to be mixed and reacted with the artemisinin acetic acid solution in advance.The ratio of each substance is L-ascorbic acid: copper sulfate: artemisinin is 24:1:2.the synthesized artemisinin-nano-copper did not precipitated out after one month at room temperature.A small amount of precipitation occur if only centrifuge at 4 ℃ 9200 r for more than 50 minutes.2.Characterization of artemisinin-nano-copper.Using transmission electron microscope(TEM),X-ray diffractometer(XRD),X-ray photoelectron spectrometer(XPS),Zeta potential,dynamic light scattering technology(DLS)to analyze the structure,morphology,element composition,The chemical valence of the elements and the stability of the nanoparticles.The results shown the synthesized nanoparticles were regular hexagons,most of the particle size range was 43.82 nm-78.82 nm,composed of Cu,O,N,C,and S five chemical elements.The copper intensity in the XRD spectrum was very high,no diffraction peaks of other substances are seen.The zeta potential(zp)value is 22.83±0.49 m V,the drift velocity(Mob)value was 1.79±0.04 μmcm/Vs,and the conductivity(Cond)was 0.65±0.01 m S/cm,the PDI value was 0.29±0.01.In addition,nanoparticles were less likely to aggregate and oxidized.3.Antibacterial properties.The sample was divided in to two groups using disk-diffusion methods.Each group was test through three quality control bacteria(E.coli ATCC 25922,Streptococcus pneumoniae ATCC 49619,and Staphylococcus aureus ATCC 29213).Results of the first group shown that the inhibition zones of artemisinin-nanocopper for Escherichia coli,Streptococcus pneumoniae and Staphylococcus aureus were 13.38±0.10 mm,14.00±0.20 mm,and 14.05±0.23 mm,respectively.The inhibition zones of artemisinin chitosan were 11.20±0.10 mm,11.10±0.10 mm,11.15±0.13 mm.The inhibition zones of chitosan nano-copper were 9.10±0.10 mm,8.55±0.09 mm,8.60±0.05 mm,respectively.The antibacterial ability was significantly different(P<0.01)between artemisinin-nano-copper,artemisinin chitosan and chitosan nano-copper.The antibacterial properties of artemisinin-nano-copper was better than artemisinin chitosan and chitosan nano-copper;The second group of results showed L-ascorbic acid do not have a effective inhibition zone,Chitosan and copper source(copper sulfate)almost have no inhibition zone.Artemisinin had a very small inhibition zone.Secondly,the growth curve test results of artemisinin-nano-copper shown it still has inhibitory effect when dilute 128 times for Escherichia coli,128 times for Streptococcus pneumoniae,and 256 times for Staphylococcus aureus(512 times for the first 10 hours).Finally,applying micro-dilution method.MIC was E.coli 0.57 mg/m L,Streptococcus pneumoniae 0.57 mg/m L,and Staphylococcus aureus 0.14 mg/m L;the MBC values was E.coli 2.29 mg/m L,Streptococcus pneumoniae 0.57 mg/m L,Staphylococcus aureus 0.28 mg/m L.Conclusion :This research determine the synthesis method of artemisinin-nano-copper,examined the morphology,chemical composition and stability of artemisinin-nano-copper,and revealed the antibacterial properties of artemisinin-nano-copper.Results shown it has better antibacterial ability than the control group.Furthermore,the results from inhibition zone,MIC and MBC,providing a new research direction for the combination of metal nanomaterials and traditional Chinese medicine.