| The plant's physiological activity and source-sink status can be reflected by cell metabolic energy.The physiological capacitance,physiological resistance and physiological impedance of the leaves from Broussonetia papyrifera and Morus alba,cultured under standard Hoagland nutrient solution,ammonium nitrogen:nitrate nitrogen 1:1 adjusted Hoagland nutrient solution and ammonium nitrogen:nitrate nitrogen 1:9 adjusted Hoagland nutrient solution,under different clamping forces were determined,and models of the physiological capacitance,physiological resistance,and physiological impedance of the leaves along with the clamping force were constructed respectively.According to the equation of Gibbs Free Energy and the Nernst equation,the metabolic energy of the plant leaf cell was jointly calculated,analysed and compared by using the parameters of the above three models.The results show that the free energy obtained based on physiological resistance calculation and impedance calculation shows no significant difference?P=0.748>0.05?.And the average free energy in leaf cells of Morus alba(8.858×10-12J)and that of Broussonetia papyrifera cells(8.486×10-12J)shows no significant difference under standard Hoagland nutrient solution culturation,indicating that the physiological activity of Morus alba and Broussonetia papyrifera in the experimental environment is quite similar,both species can grow normally.Under ammonium nitrogen:nitrate nitrogen 1:1 adjusted Hoagland nutrient solution culture conditions,the average free energy in leaf cells of Morus alba(10.148×10-12J)is lower than that of Broussonetia papyrifera cells(14.818×10-12J),indicating that the physiological activity of Broussonetia papyrifera is stronger than that of Morus alba.Under ammonium nitrogen:nitrate nitrogen 1:9 adjusted Hoagland nutrient solution culture conditions,the average free energy in leaf cells of Morus alba(7.936×10-12J)is lower than that of Broussonetia papyrifera cells(9.266×10-12J),indicating that the physiological activity of Broussonetia papyrifera is stronger than that of Morus alba.And under the above three cultivation conditions,the absolute range of free energy in leaf cells of Morus alba is smaller than that of Broussonetia papyrifera,which also reflects that the source-sink relationship between Broussonetia papyrifera leaves is more flexible than that of Morus alba.Under the standard Hogeland nutrient solution and ammonium nitrogen:nitrate nitrogen is 1:9 adjusted Hogeland nutrient solution culture conditions,the absolute range of effective specific thickness in Broussonetia papyrifera is greater than that of Morus alba,under ammonium nitrogen:nitrate nitrogen 1:1 adjusted Hoagland nutrient solution culture condition,the absolute range of effective specific thickness in Broussonetia papyrifera is smaller than that of Morus alba,indicating that the morphological diversity and functional diversity of Broussonetia papyrifera leaves are compatible.As the concentration of ammonium salt in the culture medium increased,the effective specific thickness of the leaves of Broussonetia papyrifera and Morus alba also increased,so are the physiological activities of Broussonetia papyrifera and Morus alba,and under culture condition of ammonium nitrogen:nitrate nitrogen is 1:9 adjusted Hogeland nutrient solution,that is,when the concentration of ammonium salt is low,the physiological activity of Broussonetia papyrifera is stronger than that of Morus alba,which shows that the adaptability of Broussonetia papyrifera to karst high nitrate and low ammonium nitrogen nutrition environment is stronger than that of Morus alba. |
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