| During storage,rice seeds gradually deteriorate with the increase of storage time and the change of storage environmental conditions,which affects the seed vigor and ultimately leads to the reduction of rice yield.The experiment took 271 rice core collections from 82countries as experimental materials.We selected plump-eared and pest-free matured seeds after harvested.Seeds were transferred to artificial aging cabinet at 45℃,90%humidity（RH）for 7 d and stored for 24 months under natural conditions.To select new rice core collections for breeding,we compared the germination rate in different varieties by germination test.The decrease value between the initial germination rate before aging and the germination rate after aging was used as the index to measure the storage tolerance of rice seeds.Using a genotypic data of 70K genome-wide SNPs in a sample of 271 rice core collections,we performed GWAS to locate rice seed storability-related genes.The following results are obtained:1.48 associated locations were detected by GWAS(P<10-4 level).Five storability-related SNP locations were detected on the 1、2、7th chromosome in the population under artificial aging;11 and 34 storability-related SNP locations were respectively detected in the first and second population under nature aging by TASSEl software analysis.2.In this study,candidate genes in the upper and lower 100kb regions of dd11000467（Chr11 21953047）and id11008475（Chr11 22000109）with lower P values were selected according to the GWAS test results and relevant literatures.Six candidate genes Os11g37640,Os11g37660,Os11g37700,Os11g37720,Os11g37730 and Os11g37740 were selected to design primers.6 candidate genes with high possibility of seed storability were selected Real-time quantitative PCR（RT-PCR）was used to predict whether these genes are related to storability.The results showed that the relative expression levels of Os011g37640 and Os011g37730 genes in the materials with high germination rate were lower than those of the varieties with low germination rate,and there were significant differences.These two genes may be candidate genes controlling rice seed storage tolerance at this site. |
