Effect Of Rhizosphere Oxygen Increasing On ‘Summer Black' Grape Growth And Soil Nutrients

The root respiration intensity of grape vine is high,so the oxygen content in the cultivation medium is very important for the growth and development of its root system.In this thesis,the Effects of oxygen content in soil on the growth and development of young grape trees were studied,using four oxygen increasing methods,including air pump,micro-nano bubbles,calcium peroxide and bag-controlled slow-release urea peroxide under water flooding stress,The micro-nano bubbles aeration treatment is the optimal treatment.The vine growth,plant physiology indexes and soil nutrient contents were measured,including chlorophyll content,net photosynthetic rate,leaf protective enzyme activity,root activity,soil organic matter content,soil enzyme activity,dry matter accumulation.The results shows as follows:Air pump was used to aerate the rhizosphere soil of potted grape,and four treatments were set,including aerating once every 1 day(T1),once every 3 days(T2),once every 6 days(T3),and the control(CK)without aeration.60 days after treatment,the results showed that the sucrose activity,urease activity,alkaline phosphatase activity and catalase activity in the rhizosphere soil of T2 treatment increased by 86%,56%,89% and 32%,respectively.In term of grape vine growth,the dry mass of T2 treatment was 2.3 times more than the CK.These results showed that the oxygen content and relative enzyme activity of the soil around the rhizosphere could be effectively increased by adding oxygen to the rhizosphere.2.The micro-nano bubble generator was used to oxygenate the irrigation water.The test was micro-nano bubble irrigation water(T1)and normal river water(CK).they were watered once every 6 days,and 650 ml water for each pot.The dissolved oxygen in irrigation water increased by 2.94 mg/L compared to that in conventional irrigation water for 60 min after treatment,and the oxygen-increasing treatment significantly increased leaf chlorophyll content,leaf area and net photosynthetic rate of leaves.Root activity also significantly increased.Leaf protective enzymes activitywere also significantly higher than CK.3.Grapes were cultivated in pots(inner diameter is 25 cm and depth 20 cm).Three treatments were set: 6g calcium peroxide powder(T1)per pot,8g calcium peroxide treatment(T2)per pot,and a 7 cm depth ring trench was dug in the soil.Calcium peroxide was evenly embedded and then covered with soil.No calcium peroxide was applied as control(CK).The activity of root system and activities of sucrase,urease,alkaline phosphatase and catalase in rhizosphere soil were increased.Leaf area,leaf chlorophyll content and net photosynthetic rate were significantly increased.4.A total of 4 treatments were set in the experiment: T1 bag-controlled urea peroxide treatment,T2 broadcast urea peroxide treatment,T3 broadcast ordinary urea treatment,and CK for the control without nitrogen fertilizer.The results showed that the content of dissolved oxygen in surface soil water increased after using urea peroxide.The decrease of net photosynthetic rate of grape leaves treated with urea peroxide was lower than that of CK.The content of chlorophyll a(chlorophyll a)and chlorophyll b(chlorophyll a+b)of T1 and T2 increased by 75%,69%,41%,37%,63% and 57%,respectively,compared with the CK.