Study On The Chemical Constituents Of The Root And Metabolic Differences Between Different Tissues Of Phryma Leptostachya L.

Phryma leptostachya L.is a perennial herb of the family Phrymataceae,and has been widely used as a traditional insecticidal plant in East Asia.In recent years,the pesticide research institute of northwest agriculture and forestry university has carried out a systematic study on the insecticidal active components and their mechanism of action of P.leptostachya,and found that the monomer compound,Haedoxan A?HA?,is effective against a variety of pests and has a special mechanism of action.However,the content of HA in plants is relatively low and the difficulty of chemical synthesis is great,which affects the development and utilization of HA.The rapid development of molecular biology,biochemistry,chemical analysis methods,new omics techniques and synthetic biology techniques has provided us with a new way to study the synthesis of HA.Therefore,the purpose of this study is to systematically separate the roots of the P.leptostachya,and to obtain more information about intermediate products and metabolic pathways.Then,targeted metabolomics was used to study the metabolic differences in different tissues of the plant.The aim is to lay a foundation for the future research on the synthesis of HA and to provide A theoretical basis for the further development and utilization of the plant resources.The main results are as follows:1.The roots of the P.leptostachya was extracted with methanol.Separation by gel column chromatography,high performance chromatography and recrystallization,a total of13 compounds were finally obtained from the methanol extract of the roots.By comparing the NMR data of the compounds with the data in related literature,8 compounds were identified as phrymarolin I?1?,phrymarolin II?2?,Haedoxan A?3?,54-?4,6-dimethoxy-l,3-benzodioxol-5-yl?dihydro-l-?4-methoxy-13-benzodioxol-5-yl?-l H,3H-furo[3,4-c]furan-3a?4H?-ylacetate?4?,l-?6-methoxy-l,3-benzo-dioxol-5-yl?dihydro-4-?6-methoxy-l,3-benzodioxol-5-yl?-l H,3H-fur o[3,4-c]furan-3a?4H?-yl acetate?5?,Leptostachyol acetate?6?,Haedoxan E?7?,Stigmasterol?8?.The structures of compounds 9,10,11,12 and 13 need to be further identified,which may be new compounds and further expand the number of secondary metabolites in P.leptostachya.At the same time,we combined the structural information of different compounds to predict and analyze the lignans biosynthetic metabolic pathway in the plant,which provided us with abundant metabolic pathway information for studying the biosynthetic pathway of lignans.2.The results of biossay showed that all these compounds had certain toxic activities against the 3^rd larvae of Aedes albopictus,among them compound 3,compound 4,compound 6,compound 7,compound 11,compound 12 and compound 13 showed significant toxic activities.The LC₅₀ values were 0.214?g/m L?2.849?g/m L?0.397?g/m L?3.238?g/m L?3.918?g/m L and 4.168?g/m L,respectively.3.Based on the UPLC-MS/MS metabolomics technology,the differences of lignans metabolites in different tissues and organs of P.leptostachya were studied,and the differences of metabolites in different tissues and organs of plant were preliminarily screened out,which laid a foundation for the further study of P.leptostachya lignans metabolomics.