Transcriptomic Analysis Of Elanaphis Sacchari In Response To SCYLV Infection

Sugarcane is the world's most important sugar and energy crop,featuring high photosynthetic efficiency and high biomass.Sugarcane yellow leaf disease?SCYLD?caused by yellow leaf virus?SCYLV?infection was one of the important diseases in sugarcane production.Due to the virus destroying the chloroplast structure,the photosynthetic capacity was weakened,resulting in severe reduction of sugarcane stems.After planting,it causes various degeneration of sugarcane,and breeding resistant sugarcane varieties was one of the most effective disease control measures.Since SCYLD was transmitted by aphids,And melanaphis sacchari was an important mediator of sugarcane yellow leaf virus.it was important to analyze the differential expression pattern of genes in the vector melanaphis sacchari in response to SCYLV infection,offering information in gene level for developing SCYLD-resistant varieties by molecular breeding.The main results and conclusions are as follows:1.102 636 non-repetitive sequences were obtained totally in the Melanaphis sacchari transcriptome.Based on RNA-seq analysis,there were 5,804 genes differentially expressed in the Melanaphis sacchari transcriptome data,of which 866 genes expressed up-regulatedly and4,938 genes down-regulatedly expressed.2.the differentially expressed genes were enriched into GO data.It was concluded that among the molecular functions,the binding and catalytic activity functions were most commented.Among the cell components,the cell and cell parts have the most functional annotations;in the biological process,the cellular processes and metabolic processes have the most functional annotations.The differential genes were enriched into the KEGG database,and 124 KEGG metabolic pathways were obtained in the sorghum transcriptome data,respectively.Furthermore,three metabolic pathways for salivation,basal transcription factors and biosynthesis of unsaturated fatty acids related to sugarcane yellow leaf virus infection were obtained.3.Through the analysis of sorghum transcript data,12 key genes involved in the mutual recognition of sorghum and yellow leaf virus and virus replication were screened from three metabolic pathways.Among them,11 expression genes were down-regulated and 1 expression gene was up-regulated.Among the differentially expressed genes,the ITPR1gene was a key gene in the production and transmission of Ca²⁺signals in the salivary secretory metabolic pathway.The virus was recognized by the main transduction signal Ca²⁺,which recognizes the virus.The PKG gene was a non-classical secreted protein.The replication and transport of the virus play an inhibitory role;the ATP1A and SLC12A2 genes were key genes in the transcription pathway of the basic transcription factor,and have an inhibitory effect on the transmission and replication of the virus.At the same time,SCD and desC genes play a role in the transmission of unsaturated fatty acid anabolic pathways,and have a downward trend,inhibiting the synthesis and replication of viruses by inhibiting the synthesis of unsaturated fatty acids.Among the up-regulated differentially expressed genes,the ACAA1 gene is a key gene in the unsaturated fatty acid metabolism pathway,and its high expression plays an important role in the immune regulation of the virus and inhibits viral replication.4.The results of RT-qPCR were used to verify 12 differential genes.The results showed that the differential expression of 12 differentially expressed genes in RT-qPCR was consistent with the general trend of transcriptome sequencing.The verification results indicated that the transcriptome was sequenced.The results were correct and reliable,and the validated genes provide the basis for subsequent experiments.