Effects Of Endophytic Fungi On Root Morphology And Physiological Characteristics Of Casuarina Equisetifolia Under Stress Of Two Allelochemicals

Casuarina(Casuarina equisetifolia Forst.)to evergreen trees,grow up is the south China coastal areas,the planting area is one of the most widely shelter forest tree species,in our country has important position in the process of building the coastal shelter forest,further optimize the varieties of Casuarina to guard against the typhoon and other extreme weather and slow sand invasion damage,improve the coastal ecological environment,enhance coastal shoal resources utilization is of great significance,but even plant management in long-term has seriously restricted the sustainable development of the forest of Casuarina,and because most of the varieties of Casuarina Lin is a single,The stand structure in the community is relatively simple,which leads to the poor resistance of the stand.Over time,with the growth of the forest age,the nutrients in the forest land decrease continuously,and the intra-species competition increases continuously.Previous studies have considered the factors that affect and restrict the regeneration of casuarina casuarina from the aspects of variety degradation,soil and fertilizer nutrient cycling,genetic diversity reduction under stress,disease and insect infestation aggravation,and self-poisoning stress,etc.Based on the results of previous studies,allelopathy has a significant influence on the regeneration difficulty of casuarina casuarina seedlings.Endophytic fungi,as an integral part of plant growth and development,exist in a wide range of plants and play an important role in the microecosystem of plants,and form a mutually beneficial relationship with host plants.Moreover,there may be differences in the species,quantity and activity of endophytes between different plants or different parts of the same plant,and the relationship between plants and endophytes is in long-term co-evolution.To explore the casuarinas fungus of casuarina since toxic stress relief effect and possible mechanism of cultivation area is the largest coastal casuarina in fujian "Hui An 1" clone as experiment material,with the method of hydroponic,determine CK,12.5,25,50,100,200 mg/L concentrations two short shoot casuarina equisetifolia root allelochemicals(1.Catechins;2.Effects of 3-o-carbonyl lupinol stress on root morphology and physiological characteristics of rhizome of casuarina equinis seedlings infected and uninfected with endophytic fungi.Experimental data show that endophytic fungi have a role in the growth of host,can further enhance the resistance of the plant,after the endophytic fungi infection in CK,12.5,25,50,100 and 200mg/L the 6 groups under different concentrations of catechin processing of casuarina equisetifolia root length of short shoot and can present a trend of decrease after the first increase,root surface area presents the trend of increased after decreased first,under the stress of the same concentration of catechin,above ground biomass decline is greater thanthe root biomass,root cap ratio increases,The root activity,free prolinic acid,soluble protein content,CAT content,malondialdehyde content,SOD and other physiological and biochemical indexes of EI in short shoot casuarina seedlings were increased to different degrees.In CK,12.5,25,50,100 and 200 mg/L the six groups of different concentrations of 3-O-coffee acyl lupine alcohol under duress,short shoot casuarina in endophytic fungi infection,root length,growth,root surface area were improved in different degrees,above ground biomass decline are greater than the root biomass decreased,root activity and free proline,soluble protein content,SOD,CAT content,malondialdehyde content also increase in different degrees.This indicates that in the process of plant growth,adverse conditions tend to cause plants to produce a large number of reactive oxygen species and destroy the balance of oxygen metabolism and clearance,thus causing damage to plant growth.In the face of the persecution of excessive reactive oxygen species,plants generally respond with enzymatic and non-enzymatic systems to reduce or eliminate the damage.In this study,can see clearly protective enzyme of CAT,SOD content in the activity of endophytic fungi of infection after improved,so as to speed up the role of ros,ease the allelochemicals of adverse effects on the plants,thus speculate that short shoot casuarina can react by enzyme system to help plant allelochemicals of persecution.The enzyme system the reaction of the present study also is less,can be seenfrom the experimental data,the different concentrations of catechins and3-O-coffee acyl lupine alcohol under duress,dyed bacteria short shoot casuarina seedlings in terms of root surface area,root length and biomass and dyed bacteria short shoot casuarina data produced by certain promoting effect,under the stress of two kinds of allelochemicals,dye bacterium seedling root length and root surface area than dye bacteria increased,root-shoot ratio also increased,under that allelochemicals stress,plants can also by the reaction of the enzyme system to cope with the stress of the outside world,In the future,it will become an important research direction for improving the resistance of casuarina equinis and solving the difficulty of second-generation renewal.