Effects Of Benzophenone-3 On In Vitro Cultured Mouse Sertoli Cells

Benzophenone-3(BP-3)is one of the most commonly utilized chemical compounds in personal care products which can absorb ultraviolet(UV)light.Many researches have showed that most common population is widely exposed to BP-3 and it can reside in some organs like kindey and testis via blood circulation and disrupt their physiological functions.Although it has been confirmed that BP-3 can impair spermatogenesis,its mechanism is still need to be clarified.In the present study,primary Sertoli cells isolated from 20-day-old mice testes were cultured as in vitromodel and treated with 0,5,50,100 μmol/L BP-3 for 24 h to detect its effects on Sertoli cell and Sertoli cell function including cell morphology,viability,apoptosis,transepithelial electrical resistence,cytoskeleton structure and distribution,Sertoli cell barrier function.The aim of this study is to reveal the underlying ways through which BP-3 could impair male fertility from the standpoint of Sertoli cell.Main results of the present study are as follows:After BP-3 treatment for 24 h,the morphology of Sertoli cells were changed and the cell viability was significantly decreased in 100 μmol/L group(P<0.05).Futher study showed that after BP-3 treatment at this dose,TER value was significantly decreased(P<0.05);junction proteins like ZO-1,Occludin and Connexin43 were lower expressed(p<0.01);ectoplasmic specialization protein β-catennin was mislocalized;Rictor was lower expressed(P<0.01);the distribution of F-actin was disrupted and the expression of DNA damage marker γH2AX was significantly higher(p<0.01).However,24 h BP-3 treatment could not evoke cell apoptosis.In conclusion,certain dose of BP-3 could induce the abnormalities of Sertoli cell morphology,structure and function.This could probably be the way through which BP-3 impaired male fertility.Our study expanded the knowledge of BP-3 in reproductive toxicity and partialy provided theory basis for the cure of reproductive detriments caused by BP-3 in future days.