Genome-Wide Transcriptomic Analysis Reveals The Gene Regulatory Network That Controlled By SRL1 In Regulating Rice Leaf Rolling

SRL1(SEMI-ROLLED LEAF 1)also named as CLD1(CURLED LEAF AND DWARF 1),encoding a putative glyphopholipidininol-anchored membrane protein,has been characterized as a gene involved in the regulation of leaf morphology in rice.Mutants of srl1-1(point mutation)and srl1-2(transferred DNA insertion)exhibit defects in leaf development resulting in a phenotype with adaxially rolled leaves.To explore the gene regulatory network of leaf development that controlled by SRL1 in rice,we created a homozygous SRL1 knock out(KO)line by CRISPR/Cas9,which showed defects in leaf development with adaxially rolling.By comparing the leaf transcriptome of a homozygous SRL1 KO line(srl1-KO)with the control,a total number of 3,178 genes were identified as differentially expressed genes,of which 1,216 genes were significantly up regulated,while 1,962 genes were down regulated.Further analyses indicated that,a group of known leaf rolling related genes,which involved in bulliform cells and cuticle development such as Os ZHD1、Os LBD3-7、RFS、ACL1、CFL1、SND1、Os CESA5 and Os CESA6 were up or down regulated in the srl1-KO.SRL1 might control leaf rolling by regulating a couple of genes that affecting cytological architecture of leaf cells such as bulliforms and cuticle of leaves.