Cloning Of Wheat Tae-miR0078-3p And Its Functing Under Low Phosphorus Stress

Plant microRNA(miRNA)is a kind of endogenous non coding small molecular RNA,which is involved in regulating the expression of many genes in the process of plant growth,development and metabolism.Recently,it has been found that miRNA is involved in the regulation of phosphorus uptake and utilization,which plays an important role in the adaptation of plants to low phosphorus stress.In our previous study,high-dose sequencing analysis showed that miR0078-3p was differentially expressed in the underground part of low phosphorus stress wheat(CS).In this study,wheat mir0078-3p precursor(pre-miR0078-3p)was cloned and its function was studied.The main results are as follows:The main research results are as follows:1.According to the wheat genome sequence,the sequence containing the precursor structure of Tae-miR0078-3p was cloned by PCR.2.The plant expression vector pox-pre-miR0078-3p of the gene was successfully constructed,and the antibiotic was labeled hygromycin.3.Rice plants resistant to hygromycin were obtained by Agrobacterium mediated transformation.4.Eight strains of transgenic rice with miR0078-3p gene were identified by PCR.The expression level of miR0078-3p in transgenic plants detected by qRT-PCR was significantly higher in the PCR positive plants than in the wild type.5.The expression of the predicted target gene osilr1 was analyzed.The results showed that the expression of osilr1 in transgenic rice was lower than that in wild type.6.The phenotype of transgenic rice miR0078-3p was analyzed.The results showed that the transgenic plants had higher resistance to low phosphorus stress than the wild type,and caused many other morphological and resistance gene expression changes.