Individual Variation In The Olfactory Response Of Chilo Suppressalis And Spodoptera Litura Male Moths To Sex Pheromone

The olfactory adaptability of Chilo suppressalis and Spodoptera litura affected the technical effects and strategies of field sex pheromone application.However,there are few researches on the individual differences of C.suppressalis and S.litura at home and abroad.This study compares the differences in the amount of trapped insects of C.suppressalis and S.litura males on different proportions of attractants,and to attract different hosts Differences in insect populations;Wind tunnel measurements were used to compare the differences in behavioral responses of C.suppressalis and S.litura to different ratios of attractants;RT-qPCR was used to measure odor-related genes of C.suppressalis and S.litura,and comparing Differences in olfactory gene expression between different olfactory individuals of C.suppressalis and S.litura and differences between different hosts.The main results are as following: 1.Individual variation in the olfactory responses of C.suppressalis male moths to sex pheromoneIn the field trapping experiment,attracted moth number increased with the increase of Z11-16:Ald ratio in the sex pheromone lure.The ratio of 1045: 35μg for Z11-16:Ald and Z9-16:Ald attracted the most moth(26.13%)but the ratio of 1045: 35μg attracted the lest moth(0.60%).In the wind tunnel test,the response of trapped field male moth with 864: 216μg lure to lure 864: 216μg was significantly higher than lures 980: 98μg and 1016: 64μg.The response of trapped field male moth with 1016: 64μg lure to lure 1016: 64μg was significantly higher than lures 980: 98μg and 864: 216μg.However,the response of trapped field male moth with 980: 98μg was the same to lures 1016: 64μg,980: 98μg and 864: 216μg.In the RT-qPCR assay,except GOBP1,the expression level of the other 11 sex pheromone recognition genes were significantly different among the male antennae trapped by sex pheromone lure with different ratios.In addition,there was a significant linear correlation between the expression level of genes PBP3、PBP4、PR1、PR2、PR4、PR5、PR6and the sex pheromone ratios.2.Individual variation in the olfactory responses of S.litura male moths to sex pheromoneIn the field trapping experiment,attracted moth number in decreased with the increase of Z9,E12-14:Ac ratio in the sex pheromone lure.The ratio of 1800:450μg for Z9,E11-14:Ac and Z9,E12-14:Ac attracted the most moth(20.47)but the ratio of 2077:173μg attracted the lest moth(13.55),then attracted moth number increased with the increase of Z9E12-14:Ac.In the wind tunnel test,the response of trapped field male moth with 1928: 322μg lure to lure 1928: 322μg was significantly higher than lures 1800: 450μg and 2000: 250μg.The response of trapped field male moth with 2000:250μg lure to lure 2000: 250μg was significantly higher than lures 1800: 450μg and 1928: 322μg.However,the response of trapped field male moth with 1800: 450μg was the same to lures 1800: 450μg,1928:322μg and 2000: 250μg.In the RT-qPCR assay,except PR2、PR4,the expression level of the other 6 sex pheromone recognition genes were significantly different among the male antennae trapped by sex pheromone lure with different ratios.In summary,the olfactory response of sex pheromone in the field populations of C.suppressalis and S.litura showed statistically significant differences in field trapping,wind tunnel behavior tests,and quantitative determination of related gene expressions.Through filed trapping experiment,behavior tests and quantitative determination of gene expression,combining behavior experiments and molecular experiments,it is proved by behavioral and molecular perspectives that there are individual differences in the responses of C.suppressalis and S.litura males to sex pheromone.