Development Of Compound Bacterial Fertilizer And Its Application In Chrysanthemum Morifolium Cv.BoJu

Although the traditional chemical fertilizer can guarantee the yield of plants,if it is applied for a long time,it will cause pollution to the environment and destroy the soil structure,and microbial fertilizers have obvious advantages compared to it.Endophytes were isolated and screened from the medicinal plants by using selective medium,and determine the ability of each strain to dissolve phosphorus（organic and inorganic）,potassium and cellulose.Then,it was cultured through the plate confrontation together with the functional strains stored in the laboratory to screen out strains without antagonistic effect.Making mixed bacterial suspension from the screened functional strains and using response surface method to optimize fermentation conditions to develop high efficiency solid bacterial fertilizer for BoJu.Then,pot experiments were carried out to analyze the application effect of bacterial fertilizer,which laying the foundation for the popularization and application of new compound bacterial fertilizer for BoJu.The main contents and results of this study are as follows:1.Screening of functional strains:The strains with growth promoting function were screened from endophytes isolated from medicinal plants.A total of 7 strains with no obvious antagonism were obtained by confront culture which include strains BS-a6、BS-a7、BS-a8、BS-a10、BS-a11、BN7 and BJF10.Fermentation suspension was prepared for fertilizer based on the above strains.2.Development of compound bacterial fertilizer:The physical and chemical properties of four different carriers（charcoal,peat,flower soil,peanut shell compost）and their combinations were analyzed.The results of carrier screening were as follows:the number of viable bacteria in peanut shell compost matrix was equal to that in charcoal matrix,higher than other combinations,and the adsorption capacity was stronger.In addition,the cost is low and the pollution to the environment is reduced.Therefore,peanut shell compost was selected as the carrier substrate for the development of Boju special compound bacterial fertilizer.3.Optimizing the fermentation process by using response surface method:The fermentation conditions of Boju special compound fertilizer were optimized.Based on the box benhnken sampling principle,the design of each factor level was carried out.A quadratic regression model was established with the effective number of living bacteria in the compound fertilizer as the response value.By using the design expert10.0.1 software,the optimal fermentation conditions were as follows:the ratio of water to carrier is 1.477 ml/g,the temperature is 35.137℃,and the fermentation time is 9.232 d.Under these conditions,the maximum number of viable bacteria predicted by the model is 2.643×10¹⁰cfu/g.4.Pot experiment was carried out in the greenhouse to determination of the main physiological and biochemical indicators of BoJu.The result showed that:the plant height,leaf area,number of flowers and leaves of BoJu was improved after applying the compound bacterial fertilizer.The content of chlorophyll in the leaves was increased by 61%.The content of malondialdehyde in the leaves was decreased by65%and it improves the stress resistance of BoJu.And HPLC method for determining the content of chlorogenic acid,total flavonoids,cynaroside,quercetin and luteolin in the leaves and flowers of BoJu was established.The result showed that:the contents of chlorogenic acid and total flavonoids in the leaves of BoJu was increased by 41%and 34%,respectively.The contents of chlorogenic acid,cynaroside and luteolin in the flowers of BoJu was increased by 322%,323%and50%,respectively.But the content of quercetin was almost no difference.Comprehensive analysis shows that the application of this compound bacterial fertilizer can improve the accumulation of active substances in BoJu and lay a foundation for BoJu to improve its quality and efficiency.