| Cruciferous clubroot caused by canola root fungus Plasmodiophora brassicae is a worldwide disease in cruciferae.Clubroot is a soil borne disease,plasmodiophora survives in the form of a dormant spores in soil for a long period,which is strong resistant and hard to prevent.Cruciferous crops,especially brassica crops have important economic value,such as Chinese cabbages,kales and rapeseeds are suffered from a great hazard.In recent years,it also found that plants like radix isatidis are infected by clubroot.Currently,selecting disease-resistant varieties is an effective clubroot prevention and control technology,and this is also a way mainly used in domestic.But as a result of pathogenic type diversity and genetic structure of plasmodiophora are complex,which have differentiation phenomenon of physiological races,identify the population structure of physiological races,which can provide an important theoretical basis for the cruciferous vegetables disease-resistant breeding and varieties rational layout in a certain area.According to above,this article studies on plasmodiophora collected from different disease sections in Sichuan province.The results are as follows.1.Studied on the morbidities of Chinese cabbage and arabidosis infected by the plasmodiophora spore suspension in different concentrations,and contrasted the morbidities,which found that incidence rate of NO.4 physiological race in arabidosis is slightly higher than that in Chinese cabbage.Optimized and improved Liu’s Agarose singulation technique,and arabidopsis used as host materials for inoculating the plasmodiopsis single spore and transgenerational propagation,and improved to time and effect of obtain single spore strain,which has vital significance on single spore study.2.Selected 4 pairs from 81 pairs of SRAP primers through plasmodiophora DNA amplification polymorphism,and which were F5R6,F7R5,F9R2,F9R3.These 4 pairs of primers amplified 88 bands in 72 plasmodiophora samples,and the average of bands was 22 including 81 polymorphic bands.Base on the analysis of genetic diversity,different plasmodiophora field separation strains could be separated by SRAP,but different field separation of genetic backgrounds and plasmodiophora geographical source were no significant correlation.At the same time plasmodiophora from the same field separation strains were likely to be obvious genetic variation.The molecular markers can separate part of NO.9 physiological race.3.Applying transcriptome sequencing technology obtained the roots of Chinese cabbage transcriptome data,and received a total of 13.82 G of nucleotide sequence data volume,and after the filtered clean reads respectively in 26617458(B4D_IN),30491432(B4D_CK),27569178(B10D_IN),and 25876754(B10D_CK).In the differential expression analysis,two time points of inoculation and non-inoculation treatments were screened 252 differential genes.Among them,76 genes were up-regulated gene and 77 genes were down-regulated gene.Inoculation and non-inoculation treatments after 10 days,there were 99 differential genes.Among them,37 genes were up-regulated gene and 62 genes were down-regulated gene.A total of 25 common differential genes were found on the two time points.This study selective analyzed the genes which related to hyperoxide enriched GO that the genes related to these two time points could up-regulated expression,and could be measured the contents of the materials whether the plants were stress resistance.Only chitinase appeared GO enrichment after 4 days infection,which might relate to the function of chitinase that could attack pathogens to degrade the cell wall macromolecules and release secondary elicitor.Through the analysis of secondary metabolites and phenylalanine KEGG enrichment,the mechanism of action of the two substances are likely to induce plants resistance,namely the system acquired resistance,which can help Chinese cabbage to resist plasmodiophora infection,and offer a new way for disease resistance breeding. |
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