Functional Characterization Of Nucleoporin FgNup42 In Fusarium Graminearum

Wheat scab caused by Fusarium graminearum is one of the important diseases of wheat.Wheat infected by scab produces various toxins,including Don and Zen,which seriously affect the health of human and livestock.Nuclear pore complexes are used as highly selective two-way transporters for a variety of proteins and riboproteins.However,nuclear pore complex is the only known transport pathway between nucleoplasm,but the research of nuclear pore protein in fungal diseases is very little.In this study,biological methods were used to predict the role of Nup42 gene in Fusarium graminearum.It will provide theoretical basis for the later research and development of scab control and new fungicides.In this paper,Nup42 gene of Fusarium graminearum was knocked out and supplemented by homologous recombination.According to the analysis of its biological function,the deletion of Nup42 gene results in the decrease of growth rate,increase of hyphal branches,decrease of conidia production and decrease of septum.In the stress screening test,the absence of Nup42 gene increased the resistance of Fusarium graminearum to Na~+,K~+,decreased cell wall sensitivity.The field pathogenicity test showed that the absence of Nup42 reduced the infectivity and pathogenicity of wheat ear,and the Don toxin produced by scab in wheat grain;meanwhile,the corn whisker test also proved that the infectivity of Fusarium graminearum decreased.RT-PCR was used to detect the transcription level of tri gene family in the process of inducing toxin production.Compared with wild-type PH-1,the expression of the mutant was significantly reduced.In conclusion,the results of this study indicate that Fgnup42 plays a key role in the regulation of Fusarium graminearum development,secondary metabolism and virulence.