| Genetic transformation technology has become one of the important technologies of biological research.The establishment of efficient maize genetic transformation system is of great significance to the biological research of maize.The establishment of efficient maize genetic transformation system can focus on optimizing the culture system,constructing efficient genetic transformation vector and screening efficient receptor materials and so on.64 maize inbred lines in the laboratory were transformed by Agrobacterium tumefaciens in this study.The operation steps of the sampling and infection stage refer to the most commonly used experimental methods in our laboratory.The culture system used in the experiment is suitable for most inbred lines of maize.Transformation efficiency and the culture time of obtaining regenerated seedlings were used for statistical analysis after completing genetic transformation experiment on all maize inbred lines.Three inbred lines 113,130 and 166(See Sup Tab.1 for names of inbred lines)can be used to build efficient maize genetic transformation system as receptor materials.Their transformation efficiency are 11.99%,8.78% and 9.09%,close to the control inbred lines B104 and Z31 whose transformation efficiency are 13.16% and 10.39%.The culture times of obtaining regenerated seedlings of 113,130 and 166 are 51 days,48 days and 45 days,which are shorter than that of B104 and Z31 whose culture times of obtaining regenerated seedlings are 55 days and 52 days,respectively.In this study,in order to determine the relationships between the main characters in the process of genetic transformation and transformation ability,transient transform rate,primary callus induction rate,stable transform rate and embryonic callus induction rate of all inbred lines were analyzed according to the time sequence of tissue culture.We found that(1)when primary callus induction rate or embryonic callus induction rate of a material is very low,the material can be eliminated directly;(2)when a material has a high level of primary callus induction ability or embryonic callus induction ability,it does not mean that the material has a high level of transformation efficiency;(3)the materials with low embryonic callus induction ability may have high transformation efficiency.We analyzed the influence of genotype on transient transformation ability,primary callus induction ability,stable transformation ability and embryonic callus induction ability in this study.It is found that transient transformation ability,stable transformation ability andembryonic callus induction ability are highly affected by genotype while primary callus induction ability is very lowly affected by genotype.So primary callus induction ability can no longer be used as the reference for the transformation efficiency of maize inbred lines in later experiments. |
