Preliminary Study On The Anti-oxidative Stress Effect Of Lactobacillus Plantarum And Its Mechanism

Lactobacillus plantarum,a common lactic acid bacteria,has been widely used because of its unique biological effects.As a natural non-toxic antioxidant,Lactobacillus plantarum has gradually become a research hotspot in the field of oxidative stress.In recent years,many researchers have studied the relationship between Lactobacillus plantarum and intestinal tract and the relationship between Lactobacillus plantarum and oxidative stress,and discussed the mechanism of antioxidant stress.Oxidative stress can cause the imbalance between oxidation and antioxidation in vivo thus leading to the occurrence of diseases in the body.At present,the oxidative damage of lactic acid bacteria can be reduced by reactive oxygen species(ROS)and free radical scavenging system,redox regulation system and oxidative damage repair system in the process of antioxidative damage.The antioxidative stress ability of lactic acid bacteria varies with the types of lactic acid bacteria.Lactobacillus plantarum as a potential natural antioxidant in the study of diseases caused by oxidative stress provides a new theoretical basis for the prevention and treatment of different diseases.There are few studies on the relationship between Lactobacillus plantarum and oxidative stress.Therefore,Lactobacillus plantarum NC8 and Lactobacillus plantarum NC8 pSIP409 ata-ACEIP were used to intervene in the oxidative stress model and Caenorhabditis elegans oxidative stress model.The intervention effect of Lactobacillus plantarum on oxidative stress and its mechanism of anti-oxidative stress were explored.The details are as follows:(1)The effect of Lactobacillus plantarum on oxidative stress in vitro and in vivo.Two cell lines: SH-SY5 Y cell line and APPsw cell line were used to construct an oxidative stress cell model.Different concentrations of Lactobacillus plantarum NC8 and Lactobacillus plantarum NC8 pSIP409 ata-ACEIP were used to intervene the oxidative stress cell model to find the optimal concentration.Secondly,the anti-oxidative stress of two Lactobacillus plantarum strains was determined by flow cytometry,intracellular reactive oxygen species detection(ROS)and mitochondrial membrane potential detection(MMP).The results showed that compared with oxidative stress cell model group,Lactobacillus plantarum NC8 experimental group and Lactobacillus plantarum NC8 pSIP409 ata-ACEIP experimental group showed a significant increase in the ability of antioxidant stress.The survival rates of two different cell lines increased with the increase of the concentration of the two strains of Lactobacillus plantarum.Reactive oxygen species(ROS)in the oxidative stress cell model decreased with increasing concentrations of two Lactobacillus plantarum mitochondria,and mitochondrial membrane potential(MMP)fluorescence patterns in the oxidative stress cell model followed by two Lactobacillus plantarum The concentration of the bacterial liquid increases and gradually approached the red fluorescence of normal cells.The results of flow cytometry fully demonstrated that when the concentration of Lactobacillus plantarum NC8 and Lactobacillus plantarum NC8 pSIP409 ata-ACEIP was 1.6×108 CFU/mL,the two Lactobacillus plantarum could prevent cell from oxidative stress.To further reveal the effects of Lactobacillus plantarum NC8 and Lactobacillus plantarum NC8 pSIP409 ata-ACEIP on oxidative stress,in vivo experiments were carried out with C.elegans N2 and C.elegans oxidative stress model CL2006.Through the test methods of life cycle detection,egg production detection and active oxygen detection in C.elegans,the life cycle of two strains of Lactobacillus plantarum on the oxidative stress model of C.elegans was analyzed.Whether the ability to resist oxidative stress is prolonged.The results showed that the life cycle of two different C.elegans oxidative stress models fed Lactobacillus plantarum NC8 and Lactobacillus plantarum NC8 pSIP409 ata-ACEIP could be prolonged,and the recombinant lactobacillus had the most obvious effect,but its egg production did not change significantly.The results of ROS experiments showed that the active oxygen content in the experimental group was significantly reduced compared with the negative control.(2)The mechanism of stress resistance of Lactobacillus plantarum.Western blotting was used to detect whether the two Lactobacillus plantarum cells regulate the apoptosis signal pathway by activating the ratio of Bcl-2 and Bax in the Bcl-2 family and its mechanism was analyzed.Western blot analysis showed that Lactobacillus plantarum NC8 and Lactobacillus plantarum NC8 pSIP409 ata-ACEIP could activate the ratio of Bcl-2 protein and Bax protein in the Bcl-2 protein family to inhibit apoptosis compared with the control group.Therefore,the preliminary conclusions obtained through experiments are that two Lactobacillus plantarum can effectively enhance the anti-oxidative stress of oxidative stress cell model and regulate apoptosis by regulating the ratio of Bcl-2/Bax protein.In summary,Lactobacillus plantarum NC8 and Lactobacillus plantarum NC8 pSIP409 ata-ACEIP have a certain intervention effect on the occurrence of oxidative stress by in vivo and in vitro experiments.Bcl-2/Bax signaling pathway can be activated to inhibit cell apoptosis By regulating the proportion of Bcl-2 family members.It is hoped that the research can lay a theoretical basis for future study.