Study On Root Nodule And Root Injury Characteristics Of Semi-Wild,Semi-Cultivated And Cultivated Soybean

This experiment explored the nitrogen metabolism and root injury of root nodules during the evolution of semi-wild,semi-cultivated and cultivated soybeans by comparing the nodule characteristics,root nodule nitrogen metabolism,root wound flow intensity and wound fluid composition of semi-wild,semi-cultivated and cultivated soybeans.The change of flow characteristics is aimed at finding the evolution trend of nitrogen fixation and root injury characteristics of soybean nodule,and obtaining physiological evidence for the evolution of nitrogen fixation in roots.It is expected to provide a theoretical basis for the selection and cultivation optimization of soybean varieties with high nitrogen fixation efficiency.The main results are as follows:1.The number,fresh weight and dry weight of semi-wild,semi-cultivated and cultivated soybean nodules increased to the maximum at the R6 stage,and then decreased rapidly.From R2 to R8,the number of soybeans in the three types of soybeans was fresh.And the change rule of dry weight of nodule is more than semi-cultivated and semi-wild type soybean.2.The content of hemoglobin in the three types of soybean nodules was the highest,and then decreased gradually at R6 stage.From R2 to R8,the changes of hemoglobin content in different types of soybean root nodules were the same :cultivation>semi-cultivation>semi-wild.The content of ureide in the three types of soybean nodules decreased with the growth process.From R2 to R8,the changes of the content of nodule ureide in different types of soybeans were the same as those of cultivated type and semi-cultivated and semi-wild type.From R2 to R8 stage,the free amino acid content of the three types of soybean nodules showed a downward trend,the change rule was semi-wild>semi-cultivation>cultivation,3.From R2 to R8 stage,the GS activity of the three types of soybean nodules decreased first and then increased,while the GDH activity showed a decreasing trend.In the R8 stage,the changes of GS and GDH activities of the three types of soybean nodules were observed.The regularity is cultivation>semi-cultivation>semi-wild.4.From V4 to R6,the changes of the fluid intensities and the free amino acid content of the three types of soybean root wounds showed a trend of increasing first and then decreasing,and the changes among the three were cultivation > semi-cultivation > semi-wild.From V4 to R6,the change trend of nitrogen content in the semi-wild,semi-cultivated and cultivated soybean root wound fluid is the change trend of rising-lowering,from R2 to R6,the variation law among the three is cultivation> semi-cultivation > Semi-wild.5.The content of nitrate nitrogen and ammonium nitrogen in the three types of soybean root wound fluid decreased with the progress of the growth process.The changes of nitrate nitrogen and ammonium nitrogen content from R2 to R6 phase.The regularity is semi-wild > semi-cultivation > cultivation.6.The change trend of soluble sugar and soluble protein content in the root wound fluid of semi-wild,semi-cultivated and cultivated soybeans increased firstly and then decreased with the advancement of the growth period,and the cultivated soybean root wound fluid in each growth period The content of soluble sugar and soluble protein is higher than that of semi-cultivated and semi-wild type soybean.