Molecular Function Verification Of GmLUX Regulating Soybean Growth Period

Soybean[Glycine Max(L.)Merr.]is an important cash crop.The flowering period of soybean is one of the important factors affecting the yield.It is very important to analyze the molecular mechanism of relevant genes in flowering period for improving soybean yield.It has been proved that LUX can form protein complexes with ELF3 and ELF4 in arabidopsis and other long-day plants,which is involved in the regulation of biological clock and thus affects the flowering period.However,the study on LUX gene in soybean of short-day plants is not yet complete.In this study,the analysis mechanism of two AtLUX homologous genes in soybean,Glyma.11g136600(GmLUXl)and Glyma.12g060200(GmLUX2),was preliminarily elucidated by transgenic overexpression/knockout,RT-PCR,yeast two-hybrid system and other technologies.The main research results are as follows:1.Under the condition of short day,overexpression of GmLUX1 and GmLUX2 gene could decrease the expression of El gene,increase the expression of FT2a and FT5a gene,and advance the flowering time.Knockout of GmLUX1 and GmLUX2 genes increased the expression of El gene,decreased the expression of FT2a and FT5a genes,and delayed flowering time.The expressions of GmLUXl and GmLUX2 genes in the E3 and E4 near iso-genic lines materials in Harosory's background were analyzed under short day.It was found that:E3 and E4 could inhibit to the expression of GmLUXl and GmLUX2 genes.2.The yeast two hybrid system was used to verify that:both GmLUX1 and GmLUX2 had direct interaction with J(GmELF3),but GmLUXl and GmLUX2 could not directly interact with GmELF4.In addition,six proteins interacting with GmLUX were screened from the soybean cDNA library:The result shows that GmLUX1,GmLUX2 may be related to optical system in the process of photosynthesis ?depleting the LHC's chlorophyll pigment protein complexes with relevant.The results of this experiment are helpful to improve the molecular regulation network in the flowering period of soybean and have positive significance for soybean molecular assisted breeding.