| Monochamus alternatus Hope is the main pest of Pinus kesiya Royle、Pinus massoniana Lamb and Pinus merkusii Jungh et de Vries in Vietnam,and is also the main vector of some nematodes.In this paper,Monochamus alternatus high-efficiency attractant was used to systematically investigate the population dynamics of Monochamus alternatus in Lang Son Province,Nghe An province,Thua Thien Hue province,Lam Dong province of Viet Nam and Fujian province of China.Through field trapping and indoor rearing,discussing the activity rules and life history of Monochamus alternatus in Vietnam and the morphology and genetic analysis were compared with Chinese samples.The results of this experiment provide a theoretical basis for the prevention and control of Vietnam Monochamus alternatus。The main research results was summarized as follows:1.The peak period of Monochamus alternatus adult was as follows: There are two peak periods in Dinh Lap of Lang Son Province,the first peak period was from early August to early October,and the second peak period was from mid May to mid June.Nan Dan of Nghe An Province from mid-May to mid-June.Hue of Thua Thien Hue province from early May to mid-June.Lac Duong of Lam Dong province from early May to late June.Lianjiang of Fujian from early June to late July.By comparing the population numbers of Monochamus alternatus in Liangshan of Vietnam and Fujian of China,it was found that there was no significant difference in the population numbers of Monochamus alternatus between Liangshan of Vietnam and Fujian of China,which was significantly different from the populations of Monochamus alternatus in Nghe An,Thua Thien Hue and Lam Dong province.The results of generalized linear model analysis show that various geographical factors such as altitude,latitude and longitude and tree species have significant effects on the active period of M.alternatus adult.From the results of trapping,APF-I chemical attractant can not only traped of Monochamus alternatus population,but also traped other species of the Coleoptera,Cerambycidae,and other species of the genus.2.Morphological characteristics of adult Monochamus alternatus in five different geographies were compared by three statistical methods.Cluster analysis showed that the populations in Lang Son and Thua Thien Hue of Monochamus alternatus had the closest,the smallest morphological difference and the closest genetic relationship.The morphology in Lang Son population is different from that of Lam Dong and Fujian population,and the relationship between them were far。The results of principal component analysis(PCA)were consistent with the results of cluster analysis.The results of stripe classification and genetic difference analysis indicated that the populations of M.alternatus can be divided into two subspecies,of which Lam Dong population species was a new subspecies.3.Two feeding methods(artificial feeding and semi-artificial feeding)were used to observe The developmental duration of Monochamus alternatus.The results showed that the average egg stage of artificial rearing and semi-artificial rearing were 6.75±1.055 d and 6.52±1.244 d,the average larval stage was 156.67±12.846 d and 163.1±12.773 d and the average pupae duration was 16.28±2.027 d and 17.74±1.788 d,respectively.In the results of artificial rearing,the average hatching rate was 89.47%,the pupation rate of larvae was over 84.62%,and the emergence rate was over 75%.4.Whole Genome Sequencing was used to analyze the genetic characteristics of different populations of Monochamus alternatus.The results of principal component analysis(PCA),phylogenetic tree analysis and population genetic structure analysis showed that there were significant genetic differences among different populations of Monochamus alternatus. |
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