Cloning And Function Analysis Of Glycerol-3-Phosphate Dehydrogenase(BnaGPDH) CDNA From Brassica Napus

Glycerol-3-Phosphate Dehydrogenase is a key enzyme linking lipid metabolism to glucose metabolism,GPDH catalyzes the conversion of dihydroxyacetone phosphate to glycerol 3-phosphate and NAD~+.Glycerine 3-phosphate is one of the important substrates for the synthesis of triacylglycerols.This study investigated the GPDH gene in Brassica napus.1.Through analysis of the sequenced genome of Brassica napus,three GPDH full-length coding sequences(1395,1395and1389bp)were obtained by electronic cloning,and designated as BnaGPDH1,BnaGPDH2 and BnaGPDH3,respectively.Bioinformatics analysis show that they have the characteristics of the GPDH family,and amino acid sequences of BnaGPDHs were highly homologous to GPDH in other plants.2.Spatio-temporal expression analysis showed that BnaGPDHs are constitutively expressed genes,with the highest expression in flowers,the lowest expression in leaves.BnaGPDH1 and BnaGPDH2 may play important roles in the TAG biosynthesis.BnaGPDHs responded to abiotic stresses,indicating that they may be concerned with the mechanism of plant stress resistance.3.Construction of 35S::BnaGPDH1 vector and transformation of Arabidopsis thaliana.BnaGPDH1changes the proportion ofmajor fatty acids in Arabidopsis thaliana.The proportion of C18:0 and C18:0increased by about 20%,with a net increase of 0.43%and 3.49%;the proportion of C20:1 increased by about 10%,with a net increase of 1.82%;the proportion of C16:0,C18:2,C18:3,C20:2 and C22:1increased by about-10%,with a net increase of-1.23%,-3.44%,-2.37%,-0.2%and-0.14%.