Effect Of Dark Septate Endophyte And Trichoderma Viride On The Growth And Drought Resistance Of Astragalus Membranaceus

Dark septate endophytic fungi and Trichoderma viride can promote plant growth,but there are few reports about their effects on the growth of Astragalus membranaceus.In this study,the rhizosphere soil and root samples of medicinal plants,such as Stemona japonica,Taraxacum mongolicum,Isatis indigotica and so on were collected from Anguo City,Hebei Province.DSE were isolated and identified,and DSE germplasm resources were collected.A.membranaceus was inoculated with Paraboeremia putaminum,Acrocalymma vagum conbined with T.viride which takes fermentation residue as carrier and is divided into sterilized fermentation residue(T-)and non-sterilized fermentation residue(T+).The effects of double inoculation of DSE fungi and T.viride on the growth and drought resistance of A.membranaceus were investigated.A symbiotic culture system of DSE fungi and T.viride was established to improve the yield and drought resistance of A.membranaceus and utilization rate of Chinese medicine residue.The research and application of biologic fertilizer provide material and basis.Providing materials and basis for the development and application of high-efficiency biological fertilizer.The main test results were as follows:1.DSE can infect the roots of medicinal plants such as Stemona japonica,forming a typical mycelium and micro-sclerotium structure.DSE colonization of different medicinal plants was significantly different.A total of 9 genera 10 species of DSE fungi were identified,namely Paraphoma chrysanthemicola,Paraphoma rabicina,Phoma herbarum,Alternata alternata,Fusarium acuminatum,Curvularia pallescens,Bipolaris sorokiniana.Paraboeremia putaminum,Acrocalymma vagum,Scytalidium lignicola.2.Both Paraboeremia putaminum and Acrocalymma vagum can successfully colonize in the root tissue of A.membranaceus promoting the growth and development of A.membranaceus.P.putaminum can increase the number of leaves,plant height,root dry weight and total nitrogen content of A.membranaceus,and increase soil available phosphorus and alkaline phosphatase activity;A.vagum can promote the growth of shoot,root development and biomass,total nitrogen,total Phosphorus accumulation,soil available phosphorus and alkaline phosphatase activity.T.viride increased the number of leaves,plant height,root surface area,shoot and total dry weight of the A.membranaceus and soil acid phosphatase activity.3.Compared with single inoculation,combination inoculation of T.viride and DSE had a more significant effect on the growth of A.membranaceus,soil physical and chemical properties.The mixed inoculation of P.putaminum and T.viride significantly increased the number of leaves and soil organic carbon content of A.membranaceus.A.vagum and T.viride mixed inoculation significantly increased the number of leaves,root dry weight,chlorophyll,total nitrogen,total phosphorus and soil urease activity.4.Under drought conditions,DSE significantly promoted the growth and drought resistance of A.membranaceus.DSE and T.viride alone or in combination can significantly increase the shoot dry weight,root dry weight,total dry weight and chlorophyll content of A.membranaceus,reduce leaf malondialdehyde content and peroxidase activity,and the promoting effect of DSE co-inoculating with T.viride was more significantly.A.vagum alone or in combination with T.viride significantly increased soil alkaline nitrogen content.5.Water stress significantly affects the growth and development of A.membranaceus.Drought stress significantly reduced root surface area,average root diameter,and biomass of the uninoculated plants.However,drought stress had no significant effect on root surface area,average root diameter and biomass of inoculated plants.