The Effects Of Konjac Oligosaccharides On Immune Function Of Schizothorax Prenanti

Present study was to investigate the effect of Konjac oligosaccharides on the immune system of Schizothorax prenanti.Besides,we also studied the immune-regulatory potential of Konjac oligosaccharides by challenging Schizothorax prenanti with Aeromonas hydrophila.Schizothorax prenanti were fed with diets that contain 0.4%、0.8%、1.6% KOS respectively for 8 weeks.Then they were challenged with Aeromonas hydrophila and sampled at 6h、24h、72h、168h respectively.The serum ACH50、C3、CRP、Ig M、NO、i NOS、SOD、GSH-Px、MDA activity or content,immune-related genes(TLR5、TLR22、My D88、TRAF6、NF-k B、TNF-α、IL-1β、IL-8 、 IL-10 、 β-Integrin 、 TGF-β 、 HSP70 、 CD40 、 PKR)expressions in all immune-related organs were analyzed.Results are as follow:(1)0.8% KOS significantly increased weight gain and specific growth rate of S.prenanti.(2)1.6% KOS significantly enhanced serum ACH50 activity,C3、i NOS、NO content of S.prenanti.(3)0.4%、0.8% KOS significantly enhanced serum GSH-Px content,0.4%、1.6%KOS significantly decreased serum MDA content.(4)1.6% KOS activated TLR5,TLR22 signaling pathway and finally up-regulated pro-inflammatory cytokine.Beisdes,up-regulated anti-inflammatory cytokine,which indicated that S.prenanti possessed a relatively high immunological state.(5)Aeromonas hydrophila significantly promoted immune-related parameters and immune-related genes expression,1.6% KOS can regulated the infected S.prenanti immune response and maintain it at the normal level.Therefore,1.6% KOS can activate the complement system of S.prenanti through alternative pathway;1.6% KOS can make S.prenanti possess a relatively high immunological state by activating TLR5、TLR22 signaling pathway and up-regulating anti-inflammatory cytokine;1.6% KOS can regulated the infected S.prenanti immune response and maintain it at the normal level;KOS can protect S.prenanti from oxidative damage.