| NSPs widely appear in piglets' feed,and affect the digestibility and intestinal health of piglets as an anti-nutritional factor.Supplementation of NSPases in feed is an effective way to relieve the anti-nutritional effect of NSPs,and has great significance for increasing efficiency of swine industry.The objective of the present study was to investigate the effects of NSPases(provided by AB Vista)on dietary nutrient digestion and intestinal health in piglets in vitro and in vivo.In experiment 1,After 7 days acclimatization,a total of 96 “Duroc×Landrace×Large white” piglets(7.44±0.07 kg)weaned at 21 days of age were selected,and a corn-soybean meal diet was used as the basal diet.A 2×2 factorial arrangement of treatments was used to study the effects of xylanase addition(0,30 mg/kg,i.e.0,24000 BXU/kg),mannanase addition(0,10 mg/kg,i.e.0,10000 MNU/kg),and their interactions.There were a total of 4 treatments with 8 replicates(half males and half females)each and 3 piglets in each replicate.The experiment lasted for 42 days.The results showed that supplementation of xylanase or mannanase in corn-soybean meal diet decreased the diarrhea index and the diarrhea rate of pigs in later stage(22?42d)(P < 0.05).The dry matter and Ca digestibility of pigs were increased by mannanase supplementation(P < 0.05)and the ether extract and Ca digestibility of which were increased by xylanase supplementation(P < 0.05).The finding indicated that supplementation of xylanase and mannanase could alleviate the diarrhea of piglets and improve the nutrient digestibility.In experiment 2,an in vitro simulated digestion experiment was conducted by a two-step methodology(simulating the digestion process in pig stomach and small intestine).A corn-soybean meal diet(xylan,3.33%)was formulated as the digestive substrate,and treated by 0 or 30 or 60 or 90 mg/kg(0 or 24000 or 48000 or 72000 BXU/kg)xylanase,respectively,with 6 replicates per treatment.The results showed that xylanase in corn-soybean meal diet increased IVDMD(P < 0.05)and the glucose releasing rate(P < 0.05),while the glucose releasing rate has a positive linear correlation with enzyme levels(P < 0.05).The finding indicated that,in the simulated digestion,xylanase could help hydrolyzing the cell wall and releasing more intracellular nutrient molecules,thus improved the dietary nutrient digestibility.In experiment 3,After 7 days acclimatization,a total of 54 “Duroc×Landrace×Large white” piglets(7.76 ± 0.10 kg)weaned at 21 days of age were selected,and a corn-soybean meal diet(xylan,3.33%)was used as the basal diet.This experiment was conducted as a randomized complete block design with supplementation of 0 or 30 or 60 mg/kg(0 or 24000 or 48000 BXU/kg)xylanase in diet,respectively.There were a total of 3 treatments with 6 replicates(half males and half females)each and 3 piglets in each replicate.The experiment lasted for 28 days.The results showed that supplementation of 30 mg/kg xylanase significantly increased the ADG of piglets(P < 0.05),and the addition of xylanase linearly decreased F/G of piglets(P < 0.05).Supplementation of 30 mg/kg xylanase significantly reduced the viscosity of the stomach content of piglets(P < 0.05).The gene expression of zonula occludes protein ZO-1 and inhibitor of apoptosis protein Bcl-2 were linear positive correlation with xylanase levels(P < 0.05).Xylanase linearly increased the contents of SIg A in the jejunum of piglets(P < 0.05).The finding indicated that,supplementation of xylanase in diets improved the growth performance,intestinal physical barrier and immune barrier function.In conclusion,the piglets' diarrhea could be alleviated and nutrient digestibility could be improved when 24000 BXU/kg xylanase or 10000 MNU/kg mannanase is supplemented in corn-soybean meal diets.When it comes to a corn-soybean meal diet containing 3.33% arabinoxylan,supplementation of 24000 or 48000 BXU/kg xylanase increases growth performance and has a positive influence on intestinal physical barrier and immune barrier in piglets... |
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