Genetic Variation And Tissue Expression Of PTEN Gene In Goat

PTEN（phosphate and recogniton homology does on chromosome ten）gene is the homologous gene of phosphatase and tensin on chromosome 10,belongs to the protein tyrosine phosphatase gene family.PTEN is first discovered as a tumor suppressor gene,and PTEN gene plays a role in mammalian cell growth,organ development,glucose metabolism and insulin resistance.Therefore,it is important to study on the expression and mechanism of PTEN gene in goats.In this study,the molecular characteristics of PTEN gene were investigated by analyze the coding sequence and the mutation loci of the 3’UTR in goat.We analyzed the expression of 8 tissues in goat heart,liver,spleen,lung,kidney,brain,psoas and dorsal longest muscle in E90（embryonic stage 90 th days）,E135（embryo stage 135 th day）and P90（postnatal 90 th day）,and the expression feature during the differentiation of goat precursor adipose cells.The main results are as follows:1.The coding sequence is 1212 bp in length of the PTEN gene in Jianyang Da’er goats,encoding 403 amino acids,and the GenBank access number is MG923848.PTEN is a hydrophilic protein,containing 26 phosphorylation sites and 1 signaling peptides.The results showed that the goat PTEN gene was highest homology shared with PTEN gene of ovis aries and bovine,had the lowest homology with equus caballus.In the 3’UTR of the PTEN gene,3 mutations and a 2 bp deletion were found: g.84 T>C（rs648781865）,g.996T>C（rs661678942）and unreported g.1007 C>A,g.103₁04 del AT.2.We detected that PTEN gene is expressed in Jianyang Da’er goats in E90,E135 and P90 in the heart,liver,spleen,lungs,kidney,brain,lumbar muscle and dorsal longest muscle tissue.In E90,PTEN gene was found to be predominantly expressed in liver（P<0.05）,whereas expression in the heart was relatively weak control with other tissues.In E135,the PTEN gene was expressed at significantly low levels in heart and psoas major muscle（P<0.05）than that in the other tissues and significantly higher levels in lung（P<0.05）.In P90,PTEN gene was found to be predominantly expressed in spleen（P<0.05）.The Western blotting results showed that the expression features in 8 tissues of E135 that the expression in heart,spleen and brain was significantly higher than that in other five tissues（P<0.05）,while the expression in dorsal longest muscle was significantly lower than that of 7 other tissues（P<0.05）.In addition,there is a reduction of the PTEN protein in the lung tissue of Capra hircus during the different ages.3.We also used the DAB in situ hybridization method to detected the distribution of PTEN gene in 8 tissues of Jianyang Da’er goat in P90,PTEN gene was highly expressed in liver,spleen,lung,and kidney,and was expressed lower in heart,brain,psoas and dorsal longest muscle,that was consistent with RT-qPCR results.4.According to the expression status of PTEN gene during the differentiation of goat precursor adipose cells,we found that the expression of PTEN gene showed a decreasing trend,and increased slightly in the 10 th day of differentiation.Moreover,the expression of PTEN gene was significantly higher than the other 4 time points（P<0.05）in the second day of differentiation.