Mapping Of The Genetic Male Sterility Genes In Chinese Cabbage

The Brassica cabbage of cruciferous has significant heterosis,and the use of male sterile line breeding is the most ideal method to give full play to this advantage.The Chinese cabbage bolting material used in this experiment was the sterile line of the Brassica ’ SN-1 ’,which was selected by the group,and was also the donor of the gene for the purpose of this experiment.In order to fully explore the advantages of Chinese cabbage bolting male sterile line and promote the process of Chinese cabbage bolting hybridization,this experiment,on the basis of BSR-Seq,developed SSR markers closely linked to sterile genes to locate the target genes,and obtained the following results:Prior to this test,the ’ SN-1 ’ and the DH Department of Chinese Cabbage ’ FT ’ for parents to build a breeding separation group,through the identification of F1 group traits,to judge the Chinese cabbage bolting gene and the group found in the Chinese cabbage sterile gene is not at the same point;by genetic analysis of F2 population,the sterile gene is a single gene cell nuclear male sterility gene.Through BSR-Seq technique,the male sterility gene of Chinese cabbage was initially positioned on the A01 and A05 chromosomes,and the SSR primers were designed and screened in the candidate interval by using the preliminary localization results,combined with the Chinese cabbage database,and finally obtained 2 of them,which were closely linked to the target gene: 2[12] and three[11],the Chinese cabbage genetic male sterility gene was located in the 2.99-6.5 Mb range of A01 chromosome,which contained 618 candidate genes.