Study On Rapid Propagation Technology Of Wasabi And Soilless Culture Substrate Screening

Wasabi?Wasabi japonica Matsum?is a perennial herb of the genus Brassica.The economic value of wasabi is high,but its production has high requirements for cultivation environment,long production cycle and difficult breeding.In order to solve the problems in the production of wasabi,the cultivation technology and facility cultivation of wasabi cultivation has been vigorously developed.In order to study the in vitro rapid propagation technology of wasabi and the soilless cultivation substrate of wasabi,this experiment screened the conditions of explant material,culture medium,hormone ratio and the formula of soilless culture substrate in vitro culture of wasabi.Research indicated:?1?Different organs of wasabi were used as explants,disinfected with 0.1%HgCl₂solution.The suitable disinfection time of the leaves of wasabi was 5⁸min;The suitable disinfection time of the petiole was 8¹0min.It is difficult to disinfect the buds,stems and roots.The buds need to be stripped of the outer leaves before disinfection.The suitable disinfection time was 10¹5min;the stem segments could be disinfected for 10²0min,and the suitable disinfection time was 10¹5min.The disinfection time of the wasabi seeds in the shell state was most suitable for 15 minutes,and the disinfection time after shelling was 10 minutes.?2?The most suitable explants for callus induction were young leaves,followed by functional leaves and petiole,and roots and stem segments were not suitable for callus-induced explants.?3?For the in vitro culture of wasabi tissue,MS,B5,Miller and White had the best MS in the four basic media,followed by B5 medium,and Miller and White were not suitable for in vitro culture of wasabi tissue.?4?Induction of callus by using young leaves of wasabi as explants,the most suitable medium was MS+6-BA 0.5mg/L+NAA 1 mg/L,and the callus induction rate reached 95.00%;Add 0.5mg/L 6-BA+2mg/L 2,4-D,0.1mg/L TDZ+2,4-D 2mg/L or KT0.5mg/L+2,4-D 2 mg/L to the medium,the callus induction effect is also good,the induction rate is about 90.00%.?5?It is difficult to differentiate callus into shoots.In the medium of MS+0.05mg/L NAA+0.5mg/L 6-BA/KT,a few callus differentiated into buds,and the bud induction rate was only 5.00%⁶.67%.?6?The medium with the best induction effect on the proliferation of wasabi buds was MS+0.2mg/L 6-BA+0.05mg/L NAA+0.5mg/L KT,and the bud proliferation coefficient reached 5.10;followed by 0.05mg/L NAA+0.5mg/L KT or 6-BA,and the bud proliferation coefficient was 4.78⁴.94.?7?The rooting of wasabi tissue-cultured seedlings was easier.1/2MS+0.2mg/L NAA or 1/2MS+0.5mg/L 6-BA+0.1mg/L NAA had the best effect on the root formation of wasabi seedlings,and the root formation rate reached 92.50%;1/2MS medium was more than MS medium.It is suitable for inducing root formation.The root induction rate is higher and the root activity is higher in 1/2MS medium.?8?In terms of growth,chlorophyll content,root activity and yield indicators,for soilless cultivation of wasabi,substrate?2??vermiculite:perlite=1:1?,matrix?6? ?meteorite:river sand:grass charcoal=2:1:1?>Matrix?1??meteorite:river sand=1:1?>matrix?4??meteorite:perlite:peat=1:1:1?>matrix?3??meteorite:perlite:peat=2:1:1?>Matrix?5??meteorite:river sand:grass charcoal=2:1:1?.After 10 months of soilless cultivation,the maximum fresh weight of the roots of Wasabi can reach 25.24g.