Fermented soybean meal is widely accepted around the word for better digestion efficiency than soybean meal in feed industry.However,there are some problems in the drying process,such as high cost,loss of nutrients,failure of microorganisms and active ingredients.Wet fermented soybean meal without drying process,the provincial cost and avoid the loss of nutrients,but also to ensure the activity of beneficial bacteria,with a very obvious effects and advantages,so it is significance to explore the use of wet fermented soybean meal in feed industry.This study was conducted to determine the effects of wet-fermented soybean meal on feed intake,growth performance and intestinal health in growing pigs of different stages.Healthy PIC pigs(7.0±0.5)kg,(25.0±1.0)kg,(75.0 ± 2.5)kg were randomly divided into three treatments of 10 replicates per treatment and 1 pig per replicate.Treatment A(wet fermented soybean meal),treatment B(fermented soybean meal)and treatment C(basal diet).A treatment of complex protein(10.0%)and B treatment of complex protein(10.0%)instead of 30% of the crude protein content in the basal diet,respectively.At the end of the experiment period 30 days,the feed intake of every treatment pigs was recorded.All pigs were weighed after the end of the experiment.3 pigs were randomly selected and humanely killed for each treatment.The chyme of stomach were collected for pH of the stomach.About 1~2cm duodenal was intercepted for pathological analysis the intestinal villus height and crypt depth.The results of weaning pigs showed that treatment C was significant lower than treatment A and treatment B(P <0.01),and treatment B was significant higher than treatment A(P <0.05)on Average Daily Gain(ADG).Average Daily Feed Intake(ADFI)showed that treatment A was significant higher than treatment B(P <0.05),and treatment B was significant higher than treatment C(P <0.05).Gain/Feed(G/F)showed that treatment C was significant higher than treatment A(P <0.01)and treatment B(P <0.05).The pH value of stomach chyme of treatment A was significantly lower than that of treatment C(P < 0.01)and B(P < 0.05),and treatment C was significantly higher than that of treatment B(P < 0.05).Pathological HE showed that the length of intestinal villus in treatment A and treatment B were more excellent than treatment C(P < 0.01).The depth of crypt was significantly lower than that of group C(P <0.01).The length of intestinal villus/the depth of crypt in treatment A and treatment B were significantly higher than those in treatment C(P <0.01).The results of piglets showed that treatment C was significant lower than treatment A and treatment B(P <0.01),and treatment B was significant higher than treatment A(P <0.05)on ADG.ADFI showed that treatment C was significant lower than treatment A and treatment B(P <0.01).G/F showed that treatment A was the lowest and treatment C was the highest.The pH value of stomach chyme of treatment A was significantly lower than that of treatment C(P<0.01)and B(P < 0.05),and treatment C was significantly higher than that of treatment B(P<0.05).Pathological HE showed that the length of intestinal villus in treatment A and treatment B were more excellent than treatment C(P<0.01).The depth of crypt was significantly lower than that of treatment C(P <0.01).The length of intestinal villus/the depth of crypt in treatment A and treatment B were significantly higher than those in treatment C(P <0.05).The results of growing-finishing pigs showed that treatment C was significant lower than treatment A and treatment B(P <0.01),and treatment B was significant higher than treatment A(P <0.05)on ADG.ADFI showed that treatment C was significant lower than treatment A and B(P <0.01),and treatment B was significant higher than treatment C(P <0.05).The pH value of stomach chyme of treatment A was significantly lower than that of treatment C(P<0.05).Pathological HE showed that the length of intestinal villus in treatment A were more excellent than treatment C(P<0.05).The depth of crypt was significantly lower than that of group C(P <0.05).The length of intestinal villus/the depth of crypt in treatment A were significantly higher than those in treatment C(P <0.05). |