Imazethapyr Degradating Gene Identification Of Trichoderma Brevicompactum Based On The Multi-Omics Analyses

Imazethapyr is one of the commonly used herbicides in farmland in China.Because of its good herbicidal effect and low price,it is widely used and popularized,especially in soybean fields in Heilongjiang Province.However,the long-term residue of imazethapyr makes the soil has brought certain difficulties to crop rotation and brought great pressure to the environment.Therefore,finding the mechanism of degradation of imazethapyr is crucial for solving the problem of contamination of imazethapyr.In order to study the degradation ability of Trichoderma brevicompactum fermentation broth,the culture medium was cultured in an enrichment medium,and the fermentation broths of 3,5,7,9 and 11 days were respectively taken,and the inorganic salt medium and the imazethapyr were used simultaneously.The inorganic salt medium was cultured separately for T.brevicompactum,and the different fennentation broths were separately centrifuged to obtain the supernatant of the fermentation broth,and the fermentation broth was obtained by suction filtration using a 0.22 ?m and 0.45 ?m microporous mixed membrane.The degradation rates of different fermentation broths were as follows:fermentation broth supernatant>0.22 ?m mixed membrane filtrate,fermentation broth supernatant>0.45 ?m mixed membrane filtrate,There was no significant difference with 0.45 ?m mixed membrane filtrate and 0.22 ?m mixed membrane filtrate;The degradation rate of the fermentation medium of the enriched medium was relatively high on the 7th day..In order to explore the protein expression changes and related metabolic pathways of the degradation of imazethapyr,and identify the imazethapyr degradation gene,the proteome sequencing was carried out,and the combined analysis of proteomic data and transcriptome data was completed.A total of 4003 proteins were identified in the proteome,353 differentially expressed proteins,184 proteins were up-regulated,and 169 proteins were down-regulated.Among the GO entries with significant protein enrichment,there were 195 differentially expressed proteins in the biological process,enriched in Among the 351 pathways,there were 129 differentially expressed proteins in the cell fraction,which were enriched in 62 pathways.There were 220 differentially expressed proteins in the molecular function,which were enriched in 157 pathways.The results of KEGG enrichment pathway analysis showed 258 differential proteins enriched in 105 pathways.Through proteomic analysis,the protein related to the degradation of imazethapyr can be effectively found,and the metabolic pathway can be found to provide a molecular theoretical basis for the degradation of imazethapyr.Completed correlation analysis between proteome and transcriptome data.Correlation coefficient between protein and gene expression was-0.0472,the correlation coefficient between significant difference protein and significant differential gene expression was-0.1420,and the expression change trend was the same.The correlation coefficient between protein and gene expression was-0.7413.The expression of the change trend is opposite to the protein and gene expression correlation coefficient of-0.7916;associated with 24 differentially expressed genes and protein abundance corresponding changes,wherein the expression trend is the same as 12 genes,simultaneously up-regulated 7 while down-regulating 5;There are two genes with known functions in the 7 genes of NCBInr database,namely TBU1425A and TBU3981A.After Q-pcr verification,the expression trend was consistent with the proteome and transcriptome,and the TBU1425A and TBU3981A genes were used as candidate genes for degrading imazethapyr.