Study On Biocontrol Bacteria Screening And Control Effect Of Neurospora In Edible Fungi

The harm caused by Neurospora is a very serious disease in the cultivation process of edible fungi.In order to achieve the purpose of using biological control methods to control the damage caused by Neurospora to edible fungi,the strain for biological control of Neurospora is screened in the soil.And the strain has characteristics that do not adversely affect the growth of edible fungus hypHae.The best bacteriostatic effect conditions were obtained by single factor test.extracting effective antibacterial substances with 90%ethanol solution at room temperature and testing the effect of the eluent on the degree of spore germination of Neurospora.which laid a foundation for the application of the microbial agent to the prevention and treatment of Neurospora in the production process of edible fungi.This biological control strain has not been reported.The results of this study are as follows:1?In order to screen out strains that can inhibit the pathogen of Neurospora,a strain of biological control against Neurospora was isolated from the soil under the natural forest of Pinus koraiensis in Xilinhe Forest Farm of Chaihe Forestry Bureau.This strain S-7 was subjected to molecular biology rDNA-ITS sequence analysis and identified as Xylaria polymorpha.2?Biological characteristics test results showed tha the optimum culture temperature of the strain S-7 mycelium is 25? the pH value is 6.2,the carbon source is glucose,and the nitrogen source is peptone,the best medium was the wood sawdust medium.When using strain PD to culture strain S-7,the optimal conditions for the single factor test to test the effect of strain S-7 on inhibition of Neurospora were the shaking speed of 160 r/min,the shake flask volume of 100 mL/bottle,and the optimum fermentation time of 9 d.3?Adsorption of bacteriostatic compounds using a macroporous resin of model X-5,the bacteriostatic compounds were separately eluted with 10-90%absolute ethanol.The results showed that the inhibition rate of the 80%and 90%ethanol concentration eluate was significantly higher than that of the fermentation stock solution,Among them,90%ethanol eluate had the highest inhibition rate against Neurospora mycelium,reaching 99.08%.4?In order to obtain the optimal concentration of 90%ethanol eluent,we tested the inhibitory effect of different concentrations of dilution on the spore germination of Neurospora.The results showed that The results showed that at 10 h,the 90%ethanol eluate was diluted to an effective content of 25%,and the inhibition rate of Neurospora mycelium was 36%.the 90%ethanol eluate was diluted to an effective content of 35%,and the inhibition rate of Neurospora mycelium was 92.67%.the 90%ethanol eluate was diluted to an effective content of 45%,and the inhibition rate of Neurospora mycelium was 100%.And the spore dryness was severe.It is recommended to use a 90%ethanol eluate with an effective content of 45%as a fungicide for the control of Neurospora in the production of edible fungi.5?A new method for detecting the initial extract of the fermentation broth of Xylaria polymorpha to prevent Neurospora,The method was applied to detect the control effect of the concentration of the dilution solution of the 90%ethanol concentration eluate on the germination of Neurospora crassa.during the growth of Pleurotus ostreatus mycelium.The results showed that:The 90%ethanol eluate is diluted to an effective concentration of 45%of the bacterial agent,and the amount of 400 ?L can effectively inhibit the damage of Neurospora crassa,and has no effect on the growth of mycelium and the development of fruit body.The method has the advantages of simple and easy operation and high detection efficiency.