Selection And Adaptability Analysis Of Transgenic BpCCR And BpGH3.5 Betula Platyphylla

Forest tree genetic engineering breeding technology is an effective way to quickly and efficiently cultivate fast-growing,high-resistance,and excellent material quality.The scientists use genetic engineering breeding techniques to develop the resistance and fast-growing varieties of Betula platyphylla.The research team previously obtained a batch of transgenic overexpressed and inhibited BpCCR gene Betula platyphylla^transgenic overexpressed and inhibited BpGH3.5 gene Betula platyphylla,and has entered the intermediate test,and built a transgenic intermediate test in many places.After transgenic lines have been passed through multiple generations,genetic stability analysis of the introduced target genes,comparison of growth characteristics of transgenic lines,stability and adaptability of multiple sites are important aspects of genetic engineering breeding.Therefore,this study used 7-year-old BpCCR and BpGH3.5 Betula platyphylla as materials to determine the genetic stability and growth traits of exogenous genes,and to analyze the growth stability of three sites at the same time,and select excellent transgenic lines for follow-up.The environmental release test lays the foundation and the results are as follows:(1)PCR detection showed that the exogenous target genes of BpCCR and BpGH3.5 were positive.qRT-PCR analysis showed that the BpCCR gene was up-regulated in both the sense line and the antisense line;The BpGH3.5 gene was up-regulated in the transgenic sense line,and the expression level was significantly down-regulated in the antisense line.(2)The lignin content of BpCCR positive and antisense strands increased,and the average Klason lignin and total lignin content of 10 transgenic line s increased by 7.46%and 7.05%,respectively.The FCR11 line with the highest lignin content increased by 12.26%and 11.81%,respectively.The wood density of the transgenic lines showed some changes,but there was no obvious rule.The wood fiber width of Betula platyphylla was significantly smaller,and the average value of 5 lines was 8.82%smaller than that of WT line.The wood fiber length of Betula platyphylla line was significantly inhibited,and 11 lines and WT lines were inhibited.The difference between the lines reached a significant level,and its mean was 12.12%lower than WT.(3)Determination of endogenous free IAA content showed that the IAA content of the transgenic BpGH3.5 sense line was lower or significantly lower than that of the WT line,and the five antisense lines were significantly higher than the WT line,and its IAA The average content was higher than 52.26%of the WT line.(4)The FC29 and FG12 lines have the best comprehensive evaluation,and can adapt to three test sites at the same time,and can obtain high high growth growth.The FC29 high growth was 8.67%higher than the group mean,and the genetic gain was 4.58%.The FG12 high growth was 9.49%higher than the group mean,and the genetic gain was 3.78%.The above lines provide reference for the environmental release of subsequent transgenic BpCCR and BpGH3.5 Betula platyphylla.