The Detection Of Wood Decay Fungi Based On DNA Extraction And PCR Technology

Wood decay is the gradual decomposition of the cell wall of the wood itself under the action of the fungus,which in turn leads to the appearance of spoilage or disintegration,which is mainly caused by the growth and metabolism of fungi that can invade and erode the wood.The economical use of wood is an important requirement for the effective development of circular economy and shaping into a conservation-oriented society in today’s society.In order to fundamentally solve the problem,it is necessary to find the cause of corrosion.In most cases,wood decay is caused by the growth and reproduction of related fungi that have the ability to corrode the wood itself.This fungus can be mainly divided into three categories:mold,discoloration,and wood decay.A wide variety of wood decay fungi can be divided into three types of white rot fungi,brown rot fungi and soft rot fungi.Wood white rot fungi have the largest proportion of wood decay fungi and can degrade most of the components in the wood.They are the only microorganisms in the clearly reported strains that can completely degrade lignin during pure culture.Therefore,these strains are A variety of toxic,non-degradable compounds have a very strong ability to degrade and therefore have a very significant biodegradability.The physiological metabolic process of white rot fungi is more complicated.Under suitable conditions,the growth of mycelia is more prosperous and multinuclear.Because the extraction of the fungal genome is limited by various conditions,the total genomic DNA is extracted.Methods Research is the basis for the study of the biological mechanism of the bacteria,and it can also provide assistance for later transformation and research.At the same time,the quality of DNA obtained will also influence the subsequent PCR amplification.The identification of strains by PCR technology has many advantages such as simplicity,high efficiency,accuracy,and no pollution,so it is deeply loved by experts and scholars.In this paper,wood decay fungi were firstly isolated from decaying wood,cultured and morphologically identified,and then identified by PCR in the field of molecular biology,and their comprehensive comparisons were performed.To obtain more accurate strain-related information.After research,the following results are obtained:(1)By collecting and excising the fruiting body of wood decaying fungi,disinfecting,scribing,separating and purifying them to obtain relatively pure fungi,and performing basic morphological analysis on them,the growth is fast and presents Plush and other features.(2)The whole genes of wood decay fungi were extracted by using snail enzyme method,CTAB method,SDS method,and proteinase K respectively,and the extracted DNA was subjected to ultraviolet absorption spectrum and agarose gel electrophoresis(AGE)detection,and the results were different.Comparison of the results of these methods,the experiment found that snail enzyme,CTAB,SDS extraction of DNA purity lower than the proteinase K method.One of the most effective methods for extracting total DNA of white rot fungi was the purity of DNA extracted by the proteinase K method was 1.96.(3)Amplification of PCR products using design versatility and specific primers,purification and recovery of the amplified products,DNA sequencing,comparison of relevant sequences,analysis,and observation of morphological biology Analysis,from the macro and micro levels of specific analysis,the conclusion isrthe decaying wood fungi 1 is Trametes suavelones,decaying wood fungi 2 is Trametes hirstuta.