The Development For Molecular Detection Kits Of Three Species Of Quarantine Pests

Dendroctonus valens,Lymantria dispar and Sirex noctilio are important quarantine pests worldwide,It is difficult to distinguish between morphological aspects in their larval stage.This problem,combined with traditional breeding identification method of complex and time-consuming operation,brings great difficulties to the actual inspection work.Thus,the development of efficient quarantine pest detection is of great significance for the protection of our country's ecological wellbeing.The development of molecular biology techniques make it possible to quickly and accurately identify species.At present,these techniques are widely used in the field of medicine and agriculture.The pinta disease and potato virus y molecular detection kits have been successfully developed,However,a recognized molecular identification kit was applied to the above three species of quarantine pests has not yet been reported.This experiment adopts the DNA barcoding molecular method,a selection of insect COI gene specific fragments are amplified.By using Golden Star T6 DNA polymerase,more than three species of quarantine pests can be quickly and accurately identified in 2 hours and successfully assembled into fast detection reagent boxes of three species of quarantine pests,which can evaluate the sensitivity and specificity of the kit and provide technical support for further commercial production of quarantine pest kits.